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Cupriavidus lacunae sp. nov., isolated from pond-side soil

  • Tingye Feng
  • Kyung Hyun Kim
  • Jeill Oh
  • Che Ok Jeon
Original Paper

Abstract

A Gram-stain negative, strictly aerobic, mesophilic bacterial strain, designated strain S23T, was isolated from pond-side soil of an artificial pond in South Korea. Cells were observed to be peritrichously flagellated short rods showing positive oxidase and catalase activities. Growth of strain S23T was observed at 15–37 °C (optimum, 30 °C), pH 5.0–9.0 (optimum, pH 7.0–8.0) and 0–2% (w/v) NaCl (optimum, 0–0.5%). The major respiratory quinone was identified as ubiquinone-8 and the major fatty acids were identified as C16:0, cyclo-C17:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The G + C content of the genomic DNA was determined to be 65.1 mol%. Phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid were detected as the major polar lipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain S23T formed a phyletic lineage with Cupriavidus necator N-1T within the genus Cupriavidus. Strain S23T is closely related to C. necator N-1T (99.2%), Cupriavidus basilensis DSM 11853T (98.8%), Cupriavidus alkaliphilus ASC-732T (98.8%) and Cupriavidus numazuensis TE26T (98.7%), based on 16S rRNA gene sequence similarities. However, the DNA–DNA relatedness values between strain S23T and the closely related type strains were less than 46%. On the basis of phenotypic, chemotaxonomic and molecular properties, strain S23T represents a novel species of the genus Cupriavidus, for which the name Cupriavidus lacunae sp. nov. is proposed. The type strain is S23T (KACC 19624T = JCM 32674T).

Keywords

Cupriavidus lacunae sp. nov. Taxonomy New taxa Soil 

Abbreviations

ANI

Average nucleotide identity

DDH

DNA–DNA hybridization

MP

Maximum-parsimony

ML

Maximum-likelihood

NJ

Neighbor-joining

Q-8

Ubiquinone-8

Notes

Author’s contribution

CJ conceived the ideas and supervised all works. TF isolated strain S23T from a sample and TF and KK analysed the phenotypic and biochemical properties. JO performed the genomic analysis. TF, KK, and CJ wrote the manuscript and the manuscript has been reviewed and edited by all authors.

Funding

This work was supported by Korea Environmental Industry & Technology Institute (KEITI) through the Public Technology Program based on Environmental Policy (Project No. 2016000200012) and the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR No. 2018-02-001) of the Ministry of Environment (MOE), Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants and/or animals performed by any of the authors.

Supplementary material

10482_2018_1187_MOESM1_ESM.docx (296 kb)
Supplementary material 1 (DOCX 295 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Tingye Feng
    • 1
  • Kyung Hyun Kim
    • 1
  • Jeill Oh
    • 2
  • Che Ok Jeon
    • 1
  1. 1.Department of Life ScienceChung-Ang UniversitySeoulRepublic of Korea
  2. 2.Department of Civil and Environmental EngineeringChung-Ang UniversitySeoulRepublic of Korea

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