Antagonistic yeasts from a salt-lake region in Egypt: identification of a taxonomically distinct group of phylloplane strains related to Sporisorium
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Non-pathogenic yeasts antagonising microorganisms that cause pre- and postharvest diseases of plants have been found in diverse habitats. Their practical applicability as biocontrol agents (BCAs) depends on the strength of their antagonistic activity and/or spectrum of sensitive target microorganisms. In this study, yeasts were isolated from the phylloplane and fruits of plants growing in the alkaline water lake region Wadi El-Natrun, Egypt, and tested for antifungal and antibacterial activity. All phylloplane yeast isolates belonged to the Basidiomycota and most of them could antagonise at least certain test organisms. One group of isolates showing strong antagonism against almost all fungi and yeasts appears to represent a hitherto undescribed species distantly related to the smut genus Sporisorium. This is the first report of antagonistic activity in Sporisorium. The isolates assigned to Naganishia and Papiliotrema were more effective against bacteria. The broadest range and intensity of antagonism was observed in the fruit-associated strains belonging to the ascomycetous species Wickerhamomyces subpelliculosus. The Wickerhamomyces strains are good broad-spectrum BCA candidates, the Sporisorium strains could be used as efficient antifungal BCAs, whereas the Papiliotrema isolate can be exploited as an antibacterial biocontrol agent.
KeywordsAntagonistic yeasts Antibacterial Antifungal BCA Biocontrol Desert Phylloplane yeasts
Samy A. Selim thanks Tempus Public Foundation, Hungary (Grant No. 20004) for supporting his postdoctoral research in Hungary. We thank prof. Gamal El-Din for the taxonomic identification of plants and Anita Csabai-Olah for excellent technical assistance.
MS Conceived and designed study. MS, SAS Performed research. MS Analysed data. MS Wrote the paper.
Conflict of interest
No conflict of interest to declare.
- Avis TJ, Bélanger RR (2002) Mechanisms and means of detection of biocontrol activity of Pseudozyma yeasts against plant-pathogenic fungi. FEMS Yeast Res 2:5–8Google Scholar
- Ciancio A, Pieterse CM, Mercado-Blanco J (2016) Editorial: harnessing useful rhizosphere microorganisms for pathogen and pest biocontrol. Front Microbiol 19:1620Google Scholar
- El-Banna AA, Malak AE, Shehata MG (2011) Yeasts producing killer toxins: an overview. Alex J Food Sci Technol 8:41–53Google Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Goodfellow M, Stackebrandt E (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, pp 115–175Google Scholar
- Le Mire G, Nguyen ML, Fassotte B, du Jardin P, Verheggen F, Delaplace P, Jijakli MH (2016) Review: implementing plant biostimulants and biocontrol strategies in the agroecological management of cultivated ecosystems. Biotechnol Agron Soc Environ 20(S):299–313Google Scholar
- Mari M, Di Francesco A, Bertolini P (2014) Control of fruit postharvest diseases: old issues and innovative approaches. Stewart Postharvest Rev 1:1–4Google Scholar
- O’Donell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds) The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. CAB International, Wallingford, pp 225–233Google Scholar
- Pérez-Montaño F, Alías-Villegas C, Bellogín RA, del Cerro P, Espuny MR, Jiménez-Guerrero I, López-Baena FJ, Ollero FJ, Cubo T (2013) Plant growth promotion in cereal and leguminous agricultural important plants: from microorganism capacities to crop production. Microbiol Res 169:325–336CrossRefGoogle Scholar
- Pipenberg M (2003) Smut fungi (Ustilaginomycetes p.p. and Microbotryales, Basidiomycota). Flora Neotrop 86:1–291Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Taher AG (1999) Inland saline lakes of Wadi El Natrun depression, Egypt. Int J Salt Lake Res 8:149–169Google Scholar
- White TJ, Bruns TD, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand D, Sninsky JS, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar
- Zhimo VY, Bhutia DD, Saha J, Panja B (2014) Exploitation of yeasts as an alternative strategy to control post harvest diseases of fruits. World Appl Sci J 31:785–793Google Scholar