Antonie van Leeuwenhoek

, Volume 112, Issue 4, pp 513–521 | Cite as

Mucilaginibacter formosus sp. nov., a bacterium isolated from road-side soil

  • Md. Amdadul HuqEmail author
  • Shahina Akter
  • Sun-Young LeeEmail author
Original Paper


A Gram-stain negative, aerobic, non-motile and rod-shaped novel bacterial strain, designated as MAH-5T, was isolated from a road-side soil sample and was characterised by using a polyphasic taxonomic approach. The colonies were observed to be yellowish orange in colour, smooth, circular and 0.3–0.7 mm in diameter when grown on nutrient agar for 2 days. Strain MAH-5T was found to be able to grow at 15–35 °C and at pH 4.0–8.0. The strain was observed to be positive for both the catalase and oxidase tests. Cells were found to be able to hydrolyse aesculin, gelatin and starch. By 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Mucilaginibacter and to be closely related to Mucilaginibacter panaciglaebae BXN5-31T (98.35%), Mucilaginibacter soyangensis HME6664T (97.82%), Mucilaginibacter antarcticus S14-88T (97.49%) and Mucilaginibacter ximonensis XM-003T (97.06%). In DNA–DNA hybridization tests, the DNA relatedness values between strain MAH-5T and its close phylogenetic neighbors were below 45.0%. The genomic DNA G + C content of strain MAH-5T was determined to be 41.5 mol% and the predominant isoprenoid quinine was identified as MK-7. The major fatty acids were identified as C15:0 iso and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c). The genetic characteristics, in combination with chemotaxonomic and physiological data, demonstrated that the isolated strain MAH-5T represents a novel species within the genus Mucilaginibacter, for which the name Mucilaginibacter formosus sp. nov. is proposed, with MAH-5T as the type strain (= KACC 19291T = CGMCC1.16489T).


Mucilaginibacter formosus Gram-staining negative 16S rRNA gene Fatty acid 



This study was performed with the support of the Cooperative Research Program of the National Research Foundation of Korea grant (Project No. NRF-2016R1A2B4014591) funded by the Korea government (MISP), Republic of Korea.

Author’s contributions

MAH and SYL conceived the original screening and research plans. MAH supervised the experiments and wrote the article with contributions of all the authors. MAH and SA performed all of the experiments.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1183_MOESM1_ESM.pptx (99 kb)
Supplementary Fig. S1. The maximum-likelihood (ML) tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships of strain MAH-5T and members of the genus Mucilaginibacter. Bootstrap values less than 50% are not shown (PPTX 99 kb)


  1. An DS, Yin CR, Lee ST, Cho CH (2009) Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. Int J Syst Evol Microbiol 59:1122–1125CrossRefGoogle Scholar
  2. Baik KS, Park SC, Kim EM, Lim CH, Seong CN (2010) Mucilaginibacter rigui sp. nov., isolated from wetland freshwater and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol 60:134–139CrossRefGoogle Scholar
  3. Chen X, Zhao R, Tian Y, Kong B, Li X, Chen Z, Li Y (2014) Mucilaginibacter polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum) and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol 64:1395–1400CrossRefGoogle Scholar
  4. Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354Google Scholar
  5. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
  6. Farh Mel-A, Kim YJ, Van-An H, Sukweenadhi J, Singh P, Huq MA, Yang DC (2015) Burkholderia ginsengiterrae sp. nov. and Burkholderia panaciterrae sp. nov., antagonistic bacteria against root rot pathogen Cylindrocarpon destructans, isolated from ginseng soil. Arch Microbiol 197:439–447CrossRefGoogle Scholar
  7. Fautz E, Reichenbach H (1980) A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91CrossRefGoogle Scholar
  8. Felsenstein J (1985) Confidence limit on phylogenies: an approach using the bootstrap. Evolut/Evolut Int J Org Evol 39:783–791CrossRefGoogle Scholar
  9. Gillis M, De Ley J, De Cleene M (1970) The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153CrossRefGoogle Scholar
  10. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
  11. Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469CrossRefGoogle Scholar
  12. Huq MA (2017) Chryseobacterium chungangensis sp. nov., a bacterium isolated from soil of sweet gourd garden. Arch Microbiol. Google Scholar
  13. Huq MA (2018) Caenispirillum humi sp. nov., a bacterium isolated from the soil of Korean pine garden. Arch Microbiol 200:343–348CrossRefGoogle Scholar
  14. Huq MA, Kim YJ, Min JW, Yang DC (2014) Use of Lactobacillus rossiae DC05 for bioconversion of the major ginsenosides Rb1 and Re into the pharmacologically active ginsenosides C-K and Rg2. Food Sci Biotechnol 23:1561–1567CrossRefGoogle Scholar
  15. Huq MA, Kim YJ, Hoang VA, Siddiqi MZ, Yang DC (2015) Paenibacillus ginsengiterrae sp. nov., a ginsenoside-hydrolyzing bacteria isolated from soil of ginseng field. Arch Microbiol 197:389–396CrossRefGoogle Scholar
  16. Jeon Y, Lee SS, Chung BS, Kim JM, Bae JW, Park SK, Jeon CO (2009) Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. Int J Syst Evol Microbiol 59:1451–1454CrossRefGoogle Scholar
  17. Joung Y, Joh K (2011) Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 61:1506–1510CrossRefGoogle Scholar
  18. Joung Y, Kim H, Kang H, Lee BI, Ahn TS, Joh K (2014) Mucilaginibacter soyangensis sp. nov., isolated from a lake. Int J Syst Evol Microbiol 64:413–419CrossRefGoogle Scholar
  19. Kang SJ, Jung YT, Oh KH, Oh TK, Yoon JH (2011) Mucilaginibacter boryungensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 61:1549–1553CrossRefGoogle Scholar
  20. Khan H, Chung EJ, Jeon CO, Chung YR (2013) Mucilaginibacter gynuensis sp. nov., isolated from rotten wood. Int J Syst Evol Microbiol 63:3225–3231CrossRefGoogle Scholar
  21. Kim BC, Lee KH, Kim MN, Lee J, Shin KS (2010) Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum. FEMS Microbiol Lett 309:130–135Google Scholar
  22. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefGoogle Scholar
  23. Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  24. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematic. Wiley, New York, pp 115–175Google Scholar
  25. Lee JH, Kim MS, Kang JW, Baik KS, Seong CN (2016) Mucilaginibacter puniceus sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 66:4549–4554CrossRefGoogle Scholar
  26. Lee SY, Siddiqi MZ, Kim SY, Yu HS, Lee JH, Im WT (2018) Mucilaginibacter panaciglaebae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 68:149–154CrossRefGoogle Scholar
  27. Luo X, Zhang L, Dai J, Liu M, Zhang K, An H, Fang C (2009) Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. Int J Syst Evol Microbiol 59:1447–1450CrossRefGoogle Scholar
  28. Madhaiyan M, Poonguzhali S, Lee JS, Senthilkumar M, Lee KC, Sundaram S (2010) Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. Int J Syst Evol Microbiol 60:2451–2457CrossRefGoogle Scholar
  29. Männistö MK, Tiirola M, McConnell J, Häggblom MM (2010) Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. Int J Syst Evol Microbiol 60:2849–2856CrossRefGoogle Scholar
  30. McConaughy BL, Laird CD, McCarthy BJ (1969) Nucleic acid reassociation in formamide. Biochemistry 8:3289–3295CrossRefGoogle Scholar
  31. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  32. Moore DD, Dowhan D (1995) Preparation and analysis of DNA. In: Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, New York, pp 2–11Google Scholar
  33. Pankratov TA, Tindall BJ, Liesack W, Dedysh SN (2007) Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol 57:2349–2354CrossRefGoogle Scholar
  34. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Bio Evol 4:406–425Google Scholar
  35. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI Inc, Newark, DEGoogle Scholar
  36. Stabili L, Gravili C, Tredici SM, Piraino S, Talà A, Boero F, Alifano P (2008) Epibiotic Vibrio luminous bacteria isolated from some hydrozoa and bryozoa species. Microb Ecol 56:625–636CrossRefGoogle Scholar
  37. Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849CrossRefGoogle Scholar
  38. Tamaoka J, Katayama-Fujiruma A, Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36CrossRefGoogle Scholar
  39. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. MolBiolEvol 28:2731–2739Google Scholar
  40. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefGoogle Scholar
  41. Urai M, Aizawa T, Nakagawa Y, Nakajima M, Sunairi M (2008) Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. Int J Syst Evol Microbiol 58:2046–2050CrossRefGoogle Scholar
  42. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE et al (1987) International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  43. Yoon JH, Kang SJ, Park S, Oh TK (2012) Mucilaginibacter litoreus sp. nov., isolated from marine sand. Int J Syst Evol Microbiol 62:2822–2827CrossRefGoogle Scholar
  44. Zheng R, Zhao Y, Wang L, Chang X, Zhang Y, Da X, Peng F (2016) Mucilaginibacter antarcticus sp. nov., isolated from tundra soil. Int J Syst Evol Microbiol 66:5140–5144CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Department of Food and Nutrition, College of Biotechnology and Natural ResourceChung-Ang UniversityAnseong-SiRepublic of Korea
  2. 2.Department of Horticultural Life ScienceHankyong National UniversityAnseong-SiRepublic of Korea

Personalised recommendations