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Characterization of a strain representing a new Enterobacter species, Enterobacter chengduensis sp. nov.

  • Wenjing Wu
  • Yu Feng
  • Zhiyong Zong
Original Paper
  • 18 Downloads

Abstract

An Enterobacter strain, WCHECl-C4, was recovered from a human blood sample in China in 2015. Phylogenetic analysis based on multi-locus sequence analysis of the rpoB, gyrB, infB and atpD housekeeping genes revealed that the strain is distinct from any previously described species of the genus Enterobacter. The whole genome sequence of strain WCHECl-C4 has an 80.48–93.34% average nucleotide identity with those of type strains of all known Enterobacter species. In silico DNA–DNA hybridization (isDDH) values between strain WCHECl-C4 and type strains of all known Enterobacter species range from 23.2 to 52.4%. The major cellular fatty acids of strain WCHECl-C4 are C16:0, C17:0 cyclo and C18:1ω7c, which are in the range of Enterobacter species. The genomic DNA G + C content was 55.7 mol%. Strain WCHECl-C4 can be distinguished from all known Enterobacter species by its ability to ferment d-sorbitol, l-rhamnose and melibiose but with a negative Voges–Proskauer reaction. Genotypic and phenotypic characteristics from this study indicate that strain WCHECl-C4 represents a novel species of the genus Enterobacter, for which the name Enterobacter chengduensis sp. nov. is proposed. The type strain is WCHECl-C4T (= GDMCC1.1216T = CCTCC AB 2017105T = KCTC 52993T).

Keywords

Blood Enterobacter Enterobacter chengduensis Genome 

Notes

Acknowledgements

We are grateful to Feifei Zhao and Li Wei at West China Hospital for collecting the isolate. The work was supported by grants from the National Natural Science Foundation of China (Project Nos. 81772233 and 81572030) and a joint grant from the National Natural Science Foundation of China (Project No. 81661130159) and the Newton Advanced Fellowship, Royal Society, UK (NA150363).

Authors’ contribution

WW performed the phenotype experiments; YF performed genome and phylogenetic analysis; ZZ designed the study, analyzed the data and draft the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1180_MOESM1_ESM.docx (4 mb)
Supplementary material 1 (DOCX 4105 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Center of Infectious DiseasesWest China Hospital (Huaxi)ChengduChina
  2. 2.Division of Infectious DiseasesState Key Laboratory of BiotherapyChengduChina
  3. 3.Center for Pathogen Research, West China HospitalSichuan UniversityChengduChina

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