Streptomyces monticola sp. nov., a novel actinomycete isolated from soil
- 37 Downloads
A novel actinobacterium, designated strain NEAU-GS4T, was isolated from soil collected from Mount Song and characterised using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-GS4T belongs to the genus Streptomyces, being closely related to Streptomyces spectabilis JCM 4308T (98.8%), Streptomyces sclerotialus DSM 43032T (98.3%) and Streptomyces lasiicapitis 3H-HV17(2)T (98.0%). A multilocus sequence analysis based on five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) also indicated that strain NEAU-GS4T should be assigned to the genus Streptomyces. The major menaquinones were identified as MK-9(H6), MK-9(H8) and MK-9(H4). The polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositolmannosides and an unidentified phospholipid. The major fatty acids were identified as anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Moreover, DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-GS4T can be clearly differentiated from its closely related species of the genus Streptomyces. Therefore, it is concluded that strain NEAU-GS4T represents a novel species of the genus of Streptomyces, for which the name Streptomyces monticola sp. nov. is proposed. The type stain is NEAU-GS4T (=CGMCC 4.7467T = DSM 105116T).
KeywordsStreptomyces monticola sp. nov. Polyphasic taxonomy 16S rRNA gene
This work was supported in part by grants from the National Natural Science Foundation of China (No. 31572070). We are grateful to Professor Aharon Oren for helpful advice on the specific epithet.
DL performed the laboratory experiments, analyzed the data, and drafted the manuscript. LH contributed to the biochemical characterisation. JZ contributed to the polyphasic taxonomy. HJ contributed to the morphological analyzes. SJ contributed to the fatty acids determination. XG participated in the discussions of each section of experiments. XW and WX designed the experiments and revised the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.
- Berdy J (1995) Are actinomycetes exhausted as a source of secondary metabolites? Biotechnologia 1995:13–34Google Scholar
- Collins MD (1985) Isoprenoid quinone analyses in bacterial classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic, London, pp 267–284Google Scholar
- Hatano K, Nishii T, Kasai H (2003) Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA–DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev. Int J Syst Evol Microbiol 53:1519–1529CrossRefGoogle Scholar
- Kelly KL (1964) Inter-society color council: National Bureau of standards color name charts illustrated with centroid colors. US Government Printing Office, WashingtonGoogle Scholar
- Lechevalier HA, Lechevalier MP (1970a) A critical evaluation of the genera of aerobic actinomycetes. In: Prauser H (ed) The actinomycetes. Gustav Fischer, Jena, pp 393–405Google Scholar
- Lechevalier MP, Lechevalier HA (1980) The chemotaxonomy of actinomycetes. In: Dietz A, Thayer DW (eds) Actinomycete taxonomy special publication, vol 6. Society of Industrial Microbiology, Arlington, pp 227–291Google Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654Google Scholar
- Waksman SA (1961) The actinomycetes, volume 2, classification, identification and descriptions of genera and species. Williams and Wilkins, BaltimoreGoogle Scholar
- Waksman SA (1967) The actinomycetes. A summary of current knowledge. Ronald Press, New YorkGoogle Scholar
- Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
- Wu C, Lu X, Qin M, Wang Y, Ruan J (1989) Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178Google Scholar