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Marinobacter profundi sp. nov., a slightly halophilic bacterium isolated from a deep-sea sediment sample of the New Britain Trench

  • Junwei Cao
  • Ping Liu
  • Renju Liu
  • Hainan Su
  • Yuli Wei
  • Rulong Liu
  • Jiasong Fang
Original Paper
  • 34 Downloads

Abstract

A piezotolerant, cold-adapted, slightly halophilic bacterium, designated strain PWS21T, was isolated from a deep-sea sediment sample collected from the New Britain Trench. Cells were observed to be Gram-stain negative, rod-shaped, oxidase- and catalase-positive. Growth of the strain was observed at 4–45 °C (optimum 37 °C), at pH 5.0–9.0 (optimum 7.0) and in 0.5–20% (w/v) NaCl (optimum 3–4%). The optimum pressure for growth was 0.1 MPa (megapascal) with tolerance up to 70 MPa. 16S rRNA gene sequence analysis showed that strain PWS21T is closely related to Marinobacter guineae M3BT (98.4%) and Marinobacter lipolyticus SM19T (98.2%). Multilocus sequence analysis (MLSA) based on sequences of housekeeping genes gyrB, recA, atpD, rpoB and rpoD indicates that strain PWS21T represents a distinct evolutionary lineage within the genus Marinobacter. Furthermore, strain PWS21T showed low ANI and diDDH values to the closely related species. The principal fatty acids were identified as C12:0, C12:0 3-OH, C16:1ω9c, C16:0 and C18:1ω9c. Ubiquinone-9 was identified as the major respiratory quinone. The polar lipids were identified as phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), aminophospholipid (APL), two unidentified lipids and an unidentified phospholipid (PL). The G + C content of the genomic DNA was determined to be 60.3 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, we conclude that strain PWS21T represents a novel species of the genus Marinobacter, for which the name Marinobacter profundi sp. nov. is proposed (type strain PWS21T = KCTC 52990T = MCCC 1K03345T).

Keywords

Halophilic Marinobacter profundi New Britain Trench Piezotolerant Polyphasic taxonomy 

Abbreviations

MCCC

Marine Culture Collection of China

KCTC

Korean Collection for Type Cultures

NCBI

National Center for Biotechnology Information

diDDH

The digital DNA–DNA hybridization

ANI

The average nucleotide identity

Notes

Acknowledgements

This work was supported by National Key R&D Program of China (Grant No. 2018YFC0310600) and by the National Natural Science Foundation of China (41773069, 41706146, 91328208, 41373071, and 41673085). The authors are grateful to all crews and on-board scientists of the M/V Zhangjian for taking the sediment samples in the New Britain Trench in September 2016.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1176_MOESM1_ESM.docx (2.5 mb)
Supplementary material 1 (DOCX 2541 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine SciencesShanghai Ocean UniversityShanghaiPeople’s Republic of China
  2. 2.National Engineering Research Center for Oceanic FisheriesShanghai Ocean UniversityShanghaiPeople’s Republic of China
  3. 3.Key Laboratory of Marine Genetic ResourcesThe Third Institute of State Oceanic AdministrationXiamenPeople’s Republic of China
  4. 4.State Key Laboratory of Microbial Technology, Marine Biotechnology Research CenterShandong UniversityJinanPeople’s Republic of China
  5. 5.Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoPeople’s Republic of China
  6. 6.Department of Natural SciencesHawaii Pacific UniversityHonoluluUSA

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