Marinobacter profundi sp. nov., a slightly halophilic bacterium isolated from a deep-sea sediment sample of the New Britain Trench
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A piezotolerant, cold-adapted, slightly halophilic bacterium, designated strain PWS21T, was isolated from a deep-sea sediment sample collected from the New Britain Trench. Cells were observed to be Gram-stain negative, rod-shaped, oxidase- and catalase-positive. Growth of the strain was observed at 4–45 °C (optimum 37 °C), at pH 5.0–9.0 (optimum 7.0) and in 0.5–20% (w/v) NaCl (optimum 3–4%). The optimum pressure for growth was 0.1 MPa (megapascal) with tolerance up to 70 MPa. 16S rRNA gene sequence analysis showed that strain PWS21T is closely related to Marinobacter guineae M3BT (98.4%) and Marinobacter lipolyticus SM19T (98.2%). Multilocus sequence analysis (MLSA) based on sequences of housekeeping genes gyrB, recA, atpD, rpoB and rpoD indicates that strain PWS21T represents a distinct evolutionary lineage within the genus Marinobacter. Furthermore, strain PWS21T showed low ANI and diDDH values to the closely related species. The principal fatty acids were identified as C12:0, C12:0 3-OH, C16:1ω9c, C16:0 and C18:1ω9c. Ubiquinone-9 was identified as the major respiratory quinone. The polar lipids were identified as phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), aminophospholipid (APL), two unidentified lipids and an unidentified phospholipid (PL). The G + C content of the genomic DNA was determined to be 60.3 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, we conclude that strain PWS21T represents a novel species of the genus Marinobacter, for which the name Marinobacter profundi sp. nov. is proposed (type strain PWS21T = KCTC 52990T = MCCC 1K03345T).
KeywordsHalophilic Marinobacter profundi New Britain Trench Piezotolerant Polyphasic taxonomy
Marine Culture Collection of China
Korean Collection for Type Cultures
National Center for Biotechnology Information
The digital DNA–DNA hybridization
The average nucleotide identity
This work was supported by National Key R&D Program of China (Grant No. 2018YFC0310600) and by the National Natural Science Foundation of China (41773069, 41706146, 91328208, 41373071, and 41673085). The authors are grateful to all crews and on-board scientists of the M/V Zhangjian for taking the sediment samples in the New Britain Trench in September 2016.
Conflict of interest
The authors declare that they have no conflict of interest.
- Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman J, Smith JA, Struhl K (2002) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology. Wiley, New YorkGoogle Scholar
- Dong X, Cai M (2001) Determinative manual for routine bacteriology. Scientific Press, Beijing (English translation) Google Scholar
- Luo R, Liu B, Xie Y, Li Z, Huang W, Yuan J, He G, Chen Y, Pan Q, Liu Y, Tang J, Wu G, Zhang H, Shi Y, Liu Y, Yu C, Wang B, Lu Y, Han C, Cheung DW, Yiu SM, Peng S, Xiaoqian Z, Liu G, Liao X, Li Y, Yang H, Wang J, Lam TW, Wang J (2012) SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 1:18CrossRefGoogle Scholar
- Rzhetsky A, Nei M (1992) A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967Google Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI, NewarkGoogle Scholar
- Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152Google Scholar
- Tindall B, Sikorski J, Smibert R, Krieg N (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology, vol 3. ASM Press, Washington DC, pp 330–393Google Scholar