A gene encoding a β-xylosidase (designated as Thxyl43A) was cloned from strain Thermobifida halotolerans YIM 90462T. The open reading frame of this gene encodes 550 amino acid residues. The gene was over-expressed in Escherichia coli and the recombinant protein was purified. The monomeric Thxyl43A protein presented a molecular mass of 61.5 kDa. When p-nitrophenyl-β-d-xylopyranoside was used as the substrate, recombinant Thxyl43A exhibited optimal activity at 55 °C and pH 4.0 to 7.0, being thermostable by maintaining 47% of its activity after 30 h incubation at 55 °C. The recombinant enzyme retained more than 80% residual activity after incubation at pH range of 4.0 to 12.0 for 24 h, respectively, which indicated notable thermostability and pH stability of Thxyl43A. Moreover, Thxyl43A displayed high catalytic activity (> 60%) in presence of 5–35% NaCl (w/v) or 1–20% ionic liquid (w/v) or 1–50 mM xylose. These properties suggest that Thxyl43A has potential for promoting hemicellulose degradation and other industrial applications.
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This research was supported by Infrastructure work project of China Ministry of Science and Technology (No. 2015FY110100), Guangzhou Municipal People’s Livelihood Science and Technology Plan (No. 201803030030), the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant No. (RGP-1438-0004) and China Postdoctoral Science Foundation (Grant No. 2017M622861). W-J Li is supported by project funded by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).
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Yin, Y., Xian, W., Han, M. et al. Expression and characterisation of a pH and salt tolerant, thermostable and xylose tolerant recombinant GH43 β-xylosidase from Thermobifida halotolerans YIM 90462T for promoting hemicellulose degradation. Antonie van Leeuwenhoek 112, 339–350 (2019) doi:10.1007/s10482-018-1161-2
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