Antonie van Leeuwenhoek

, Volume 112, Issue 2, pp 159–165 | Cite as

Marinicaulis aureum sp. nov., isolated from a culture of the green alga Ulva prolifera, and emended description of the genus Marinicaulis

  • Zengmeng Wang
  • Zenghu ZhangEmail author
  • Chengcheng Li
  • Zhenzhen Hu
  • Hanshuang Zhao
  • Yongyu Zhang
Original Paper


A Gram-stain negative, facultatively anaerobic, rod-shaped motile bacterium with a single polar flagellum, designed strain HHTR114T, was isolated from a culture of the green alga Ulva prolifera obtained from offshore seawater at Qingdao, China. Optimum growth occurred in the presence of 2–3% (w/v) NaCl, at pH 7.0–8.0 and 30 °C. The major fatty acids (> 10% of total fatty acids) were C16:0 (24.7%), C18:1ω7c 11-methyl (24.3%) and summed feature 3 (C16:1ω6c and/or C16:1ω7c, 19.7%). The major polar lipids were phosphatidylethanolamine, glycolipid and four unidentified polar lipids. The DNA G + C content of strain HHTR114T calculated on the basis of the genome sequence was 58.2% and the genome size is 4.1 Mbp. The predominant isoprenoid quinone was Q-10. The estimated DNA–DNA hybridization values were 21.4% [18.6–24.4%] between strain HHTR114T and Marinicaulis flavus SY-3-19T. On the basis of polyphasic analysis, strain HHTR114T is considered to represent a novel species, for which the name Marinicaulis aureum sp. nov. is proposed. The type strain of the type species is HHTR114T (= KCTC 62394T = MCCC 1K03481T).


Polyphasic taxonomy Marinicaulis aureum sp. nov. 16S rRNA gene Ulva prolifera 



We are grateful to the Japan Collection of Microorganism and the Korean Collection for Type Cultures for providing the reference type strains.


This work was supported by the open task of Qingdao National Laboratory for Marine Science and Technology (QNLM2016ORP0311), the National Key Research and Development Program of China (2016YFA0601402), the CNOOC Tianjin project (CNOOC-KJ 125 FZDXM 00TJ 001-2014) and Natural Science Foundation of China (No. 31700104). This study is a contribution to the international IMBER project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Supplementary material

10482_2018_1139_MOESM1_ESM.docx (769 kb)
Supplementary material 1 (DOCX 769 kb)


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© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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