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Jiangella anatolica sp. nov. isolated from coastal lake soil

  • Hilal AyEmail author
  • Imen Nouioui
  • Lorena Carro
  • Hans-Peter Klenk
  • Demet Cetin
  • José M. Igual
  • Nevzat Sahin
  • Kamil Isik
Original Paper
  • 60 Downloads

Abstract

A novel actinobacterial strain, designated GTF31T, was isolated from a coastal soil sample of Gölcük Lake, a crater lake in southwest Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences and showed that the strain is closely related to Jiangella gansuensis DSM 44835T (99.4%), Jiangella alba DSM 45237T (99.3%) and Jiangella muralis DSM 45357T (99.2%). Optimal growth was observed at 28 °C and pH 7–8. Whole cell hydrolysates were found to contain LL-DAP, glucose, mannose, rhamnose and ribose. The predominant menaquinone was identified as MK-9(H4). The polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycophospholipids and unidentified phospholipids. The major fatty acids were identified as anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The G + C content of the type strain was determined to be 72.5% and the size of the draft genome is 7.0 Mb. The calculated digital DDH values between strain GTF31T and the type strains of J. gansuensis, J. alba, J. muralis and Jiangella alkaliphila ranged from 24.4 to 34.4% and ANI values ranged between 81.0 and 87.9%. Based upon the consensus of phenotypic and phylogenetic analyses as well as whole genome comparisons, strain GTF31T (= DSM 100984T = CECT 9378T) is proposed to represent the type strain of a novel species, Jiangella anatolica sp. nov.

Keywords

Phylogenomic Gölcük Lake Jiangella Polyphasic taxonomy 

Notes

Acknowledgements

HA is grateful for the Scientific and Technological Research Council of Turkey (TÜBİTAK) for the Ph.D. scholarship. IN and LC are grateful for Newcastle University for the postdoctoral fellowship.

Author contributions

HA, NS and KI designed the study. HA, IN and LC conducted physiological experiments. HPK, HA, IN, JMI designed chemotaxonomic analyses. DC carried out scanning electron microscopy analysis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Supplementary material

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Supplementary material 1 (DOCX 338 kb)
10482_2018_1222_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 15 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Molecular Biology and Genetics, Faculty of Science and ArtsOndokuz Mayis UniversitySamsunTurkey
  2. 2.School of Natural and Environmental SciencesNewcastle UniversityNewcastle upon TyneUK
  3. 3.Dpto. de Microbiología y GenéticaUniversidad de SalamancaSalamancaSpain
  4. 4.Science Teaching Programme, Gazi Faculty of EducationGazi UniversityAnkaraTurkey
  5. 5.Instituto de Recursos Naturales y Agrobiologia de Salamanca, Consejo Superior de Investigaciones Cientificas (IRNASA-CSIC)SalamancaSpain
  6. 6.Department of Biology, Faculty of Science and ArtsOndokuz Mayis UniversitySamsunTurkey

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