Ethanol stress responses of Kluyveromyces marxianus CCT 7735 revealed by proteomic and metabolomic analyses

  • Mariana Caroline Tocantins Alvim
  • Camilo Elber Vital
  • Edvaldo Barros
  • Nívea Moreira Vieira
  • Fernando Augusto da Silveira
  • Thércia Rocha Balbino
  • Raphael Hermano Santos Diniz
  • Amanda Fernandes Brito
  • Denise Mara Soares Bazzolli
  • Humberto Josué de Oliveira Ramos
  • Wendel Batista da SilveiraEmail author
Original Paper


Kluyveromyces marxianus CCT 7735 offers advantages to ethanol production over Saccharomyces cerevisiae, including thermotolerance and the ability to convert lactose to ethanol. However, its growth is impaired at high ethanol concentrations. Herein we report on the protein and intracellular metabolite profiles of K. marxianus at 1 and 4 h under ethanol exposure. The concentration of some amino acids, trehalose and ergosterol were also measured. We observed that proteins and metabolites from carbon pathways and translation were less abundant, mainly at 4 h of ethanol stress. Nevertheless, the concentration of some amino acids and trehalose increased at 8 and 12 h under ethanol stress, indicating an adaptive response. Moreover, our results show that the abundance of proteins and metabolites related to the oxidative stresses responses increased. The results obtained in this study provide insights into understanding the physiological changes in K. marxianus under ethanol stress, indicating possible targets for ethanol tolerant strains construction.


Amino acids Trehalose Ergosterol Membrane permeability Lactose 



This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Besides, this study was supported by the Brazilian Agencies Foundation for Research Support of the State of Minas Gerais (FAPEMIG) as well as National Science and Technology Development Council (CNPq). The authors thank the Center for Analysis of Biomolecules at Universidade Federal de Viçosa for the equipment’s and software’s used in this study.

Author’s contribution

Considering the involvement, we believe that it is appropriate to include the following authors in the manuscript: MCTA, CEV, EB, NMV, FAS, TRB, RHSD, AFB, DMSB, HJOR, WBS. Conceived of or designed study: WBS, HJOR and MCTA. Performed research: MCTA, CEV, EB, NMV, FAS, TRB, RHSD and AFB. Analyzed data and discussion relative: WBS, MCTA, CEV and EB. Wrote and revised the manuscript: WBS, FAS, DMSB and MCTA.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1214_MOESM1_ESM.docx (750 kb)
Supplementary material 1 (DOCX 750 kb)


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Mariana Caroline Tocantins Alvim
    • 1
  • Camilo Elber Vital
    • 2
  • Edvaldo Barros
    • 2
  • Nívea Moreira Vieira
    • 1
    • 2
  • Fernando Augusto da Silveira
    • 1
  • Thércia Rocha Balbino
    • 1
  • Raphael Hermano Santos Diniz
    • 1
    • 3
  • Amanda Fernandes Brito
    • 1
  • Denise Mara Soares Bazzolli
    • 1
  • Humberto Josué de Oliveira Ramos
    • 2
  • Wendel Batista da Silveira
    • 1
    Email author
  1. 1.Laboratory of Microbial Physiology, Department of MicrobiologyUniversidade Federal de ViçosaViçosaBrazil
  2. 2.Center for Analysis of Biomolecules, Center for Biological and Health SciencesUniversidade Federal de ViçosaViçosaBrazil
  3. 3.Instituto Federal de EducaçãoCiência e Tecnologia de Minas Gerais - Campus Ouro PretoOuro PretoBrazil

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