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Lottiidibacillus patelloidae gen. nov., sp. nov., isolated from the intestinal tract of a marine limpet and reclassification of Bacillus taeanensis as Maribacillus taeanensis gen. nov., comb. nov.

  • Renju Liu
  • Zhaobin Huang
  • Chunming Dong
  • Zongze Shao
Original Paper
  • 16 Downloads

Abstract

A taxonomic study was carried out on strain SA5d-4T, which was isolated from a marine limpet (Patelloida saccharina lanx [Reeve, 1855]) collected from intertidal rocks in Xiamen, China. Strain SA5d-4T was aerobic, Gram-positive, lacked flagellum, non-motile, filamentous, formed a slightly-yellowish colony, and non-sporulating. The strain grew optimally at 28 °C, at pH values 7.0–8.0, and in the presence of 1–2% (w/v) sodium chloride. The major cellular fatty acids identified were iso-C15:0, iso-C17:0ω10c, and iso-C17:0. The dominated respiratory quinone was menaquinone-7. The major phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G + C content was 35.3 mol%, calculated from a draft genome sequence. Phylogenetic analysis based on the full-length 16S rRNA gene sequence showed that strain SA5d-4T belongs to a new genus within the family Bacillaceae, and this gene shares 95.6% similarity with that from Bacillus taeanensis BH030017T, 95.2% with Bacillus algicola KMM 3737T, 95.1% with Bacillus alkalinitrilicus ANL-iso4T, 94.9% with Bacillus hwajinpoensis SW-72T, and 94.6% with Anaerobacillus alkalidiazotrophicus MS6T. Whole genome phylogenetic analyses indicated that strain SA5d-4T formed a monophyletic branch with B. taeanensis BH030017T. The average nucleotide identity between strain SA5d-4T and B. taeanensis BH030017T was 69.6%. Based on polyphasic taxonomic characteristics, strain SA5d-4T represents a novel species of a new genus, for which the name Lottiidibacillus patelloidae gen. nov., sp. nov., is proposed with the type strain SA5d-4T (= MCCC 1A11654T = KCTC 33831T). Based on phylogenetic analyses, B. taeanensis should be transferred to a new genus, named Maribacillus, as Maribacillus taeanensis comb. nov., with type strain BH030017T (= KCTC 3918T = DSM 16466T).

Keywords

Lottiidibacillus patelloidae Marine limpet Taxonomy Maribacillus taeanensis Reclassification 

Abbreviations

MCCC

Marine Culture Collection of China

KCTC

Korean Collection for Type Cultures

JCM

Japan Collection of Microorganisms

Notes

Acknowledgements

We thank Professor A. Oren (The Hebrew University of Jerusalem) for his suggestion regarding Latin nomenclature for the new genera. And we are grateful to KCTC and JCM for their kind providing the reference type strains. This work was financially supported by the projects of the National Natural Science Foundation of China (No. 41676149) and National Infrastructure of Microbial Resources of China (NIMR-2018-9). We also thank James Allen, DPhil, from Liwen Bianji, Edanz Group China, for editing the English text of a draft of this manuscript.

Conflicts of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1213_MOESM1_ESM.doc (3.3 mb)
Supplementary material 1 (DOC 3400 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Renju Liu
    • 1
    • 2
  • Zhaobin Huang
    • 1
    • 2
  • Chunming Dong
    • 1
    • 2
  • Zongze Shao
    • 1
    • 2
  1. 1.State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, State Oceanic Administration; Fujian Key Laboratory of Marine Genetic ResourcesThird Institute of Oceanography, SOAXiamenChina
  2. 2.Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological ResourcesXiamenChina

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