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Pseudonocardia tritici sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.)

  • Jia Song
  • Shiwen Qiu
  • Junwei Zhao
  • Chuanyu Han
  • Ying Wang
  • Xiujun Sun
  • Shanwen Jiang
  • Xiangjing Wang
  • Wensheng Xiang
Original Paper
  • 12 Downloads

Abstract

An aerobic, non-motile, Gram-stain positive actinomycete, designated strain NEAU-YY211T, was isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, mid-eastern China, and characterised taxonomically using a polyphasic approach. Comparative analysis of the 16S rRNA gene sequence indicated that strain NEAU-YY211T belongs to the genus Pseudonocardia, showing high similarities with respect to Pseudonocardia ammonioxydans H9T (99.1%) and Pseudonocardia antitumoralis SCSIO 01299T (99.0%), respectively. The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as arabinose and galactose. The predominant menaquinone of strain NEAU-YY211T was identified as MK-8(H4) and the major fatty acids were identified as iso-C16:0, C17:1ω8c and iso-C16:1. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unidentified phospholipid. The G+C content of the genomic DNA was determined to be 72.6 mol%. Levels of DNA–DNA relatedness with P. ammonioxydans JCM 12462T and P. antitumoralis DSM 45322T were 54.5 ± 3.5% and 49.8 ± 2.5% (mean ± SD), respectively. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate can be distinguished from closely related type strains and thus represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia tritici sp. nov. is proposed. The type strain is NEAU-YY211T (= DSM 106068T = CGMCC 4.7474T).

Keywords

Pseudonocardia tritici sp. nov. Polyphasic analysis 16S rRNA gene Phenotypic characteristics 

Notes

Acknowledgements

This work was supported in part by grants from the National Natural Science Foundation of China (No. 31700067), the Natural Science Foundation of Heilongjiang Province (LC2016011), the ‘Young Talents’ Project of Northeast Agricultural University (14QC04), Key Laboratory of Agricultural Microbiology of Heilongjiang Province (NW2017001), the China Postdoctoral Science Foundation (2015M580255) and the Heilongjiang Provincial Postdoctoral Science Foundation (LBH-Z15016). We are grateful to Professor Aharon Oren for helpful advice on the specific epithet.

Authors’ contribution

JS performed the laboratory experiments, analysed the data, and drafted the manuscript. SQ contributed to the biochemical characterisation. JZ and YW contributed to the polyphasic taxonomy. CH contributed to the morphological analyses. SJ and XS contributed to the fatty acids determination. XW and WX designed the experiments and revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Supplementary material

10482_2018_1210_MOESM1_ESM.docx (2.4 mb)
Supplementary material 1 (DOCX 2412 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Jia Song
    • 1
  • Shiwen Qiu
    • 1
  • Junwei Zhao
    • 1
  • Chuanyu Han
    • 1
  • Ying Wang
    • 1
  • Xiujun Sun
    • 1
  • Shanwen Jiang
    • 1
  • Xiangjing Wang
    • 1
  • Wensheng Xiang
    • 1
    • 2
  1. 1.Key Laboratory of Agricultural Microbiology of Heilongjiang ProvinceNortheast Agricultural UniversityHarbinPeople’s Republic of China
  2. 2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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