A role for the endometrial microbiome in dysfunctional menstrual bleeding

  • Elise S. Pelzer
  • Dana Willner
  • Melissa Buttini
  • Flavia Huygens
Original Paper

Abstract

This study aimed to characterise the microbial community within the endometrial cavity and endocervix in women with menorrhagia or dysmenorrhea. Paired endocervical and endometrial biopsy samples were collected from women undergoing operative hysteroscopy and/or laparoscopy. Samples were cohorted based on pathology, indications for surgery, and histological dating of the endometrium. Samples were interrogated for the presence of microbial DNA using a two-step next generation sequencing technology approach to exploit the V5–V8 regions of the 16S rRNA gene. Pyrosequencing revealed that the endocervix and endometrium share a minor microbial community, but that each site harbours a separate and distinct microbial population (p = 0.024). This was also the case for women with menorrhagia and dysmenorrhea (p = 0.017). Lactobacillus spp. were the most abundant microbial taxa present in 50% of the cohorts, and across all endocervical groups. Members of the genera Prevotella, Fusobacterium and Jonquetella were the most abundant taxa identified in samples collected from nulliparous women. It can be concluded that the female upper genital tract is not sterile. Microbial community profiling revealed differences in the endometrial microbial community profiles for: (1) the endocervix compared to the endometrium, and (2), women with menorrhagia versus dysmenorrhea. The distinct microbial community profiles in these women may offer insight into the pathology and clinical management of dysfunctional menstrual bleeding.

Keywords

Menorrhagia Dysmenorrhea Microbial community profile Lactobacillus sp. Endometrium 

Notes

Acknowledgements

The authors wish to thank Wesley Hospital theatre staff who facilitated collection of the genital tract samples. We wish to acknowledge the Australian Centre for Ecogenomics for 454 pyrosequencing and Professor Philip Hugenholtz and Dr Fiona May. This work was performed in the Thorsen Group Women’s Health Laboratory.

Compliance with ethical standards

Conflict of interest

No competing financial interests exist.

Ethical approval

All procedures performed in studies involving human participant were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Health, School of biomedical SciencesQueensland University of TechnologyBrisbaneAustralia
  2. 2.Women’s Health Laboratory, The Wesley Research InstituteThe Wesley HospitalAuchenflowerAustralia
  3. 3.Australian Centre for EcogenomicsUniversity of QueenslandSt LuciaAustralia
  4. 4.The Wesley HospitalAuchenflowerAustralia

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