Rhodococcus psychrotolerans sp. nov., isolated from rhizosphere of Deschampsia antarctica
- 336 Downloads
A novel actinobacterium, designated strain CMAA 1533T, was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533T was found to grow over a wide range of temperatures (4–28 °C) and pH (4–10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533T belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus. Sequence similarity calculations indicated that the novel strain is closely related to Rhodococcus degradans CCM 4446T, Rhodococcus erythropolis NBRC 15567T and Rhodococcus triatomae DSM 44892T (≤ 96.9%). The organism was found to contain meso-diaminopimelic acid, galactose and arabinose in whole cell hydrolysates. Its predominant isoprenologue was identified as MK-8(H2) and the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids were identified as Summed feature (C16:1 ω6c and/or C16:1 ω7c), C16:0, C18:1 ω9c and 10-methyl C18:0. The G+C content of genomic DNA was determined to be 65.5 mol%. Unlike the closely related type strains, CMAA 1533T can grow at 4 °C but not at 37 °C and was able to utilise adonitol and galactose as sole carbon sources. Based on phylogenetic, chemotaxonomic and physiological data, it is concluded that strain CMAA 1533T (= NRRL B-65465T = DSM 104532T) represents a new species of the genus Rhodococcus, for which the name Rhodococcus psychrotolerans sp. nov. is proposed.
KeywordsAntarctic actinomycetes CMAA 1533T Polyphasic approach Taxonomy
Silva, L.J. thanks the support from National Council for Scientific and Technological Development [CNPq 141705/2014-0] and [CNPq PROANTAR 407230/2013-0, INCT Criosfera]. Genuario, D. B. and Souza, D.T were supported by FAPESP graduate scholarships 2014/26131-7 and 2013/25505-8, respectively. The authors are grateful to Marcia Maria Parma Leme, João Luiz da Silva, Renato Barbosa Salaroli and Roseli dos Santos Nascimento by their contributions with the laboratory techniques. Finally, thanks are due to the PROANTAR Research Program and Brazilian Navy for logistical support during the OPERANTAR EXPEDITION.
Conflict of interest
The authors declare that they have no conflict of interest.
- Jones AL, Goodfellow M (2015) Rhodococcus Bergey´s manual of systematics bacteriology. Springer, BerlinGoogle Scholar
- Jones AL, Sutcliffe IC, Goodfellow M (2013) Proposal to replace the illegitimate genus name Prescottia Jones et al. 2013 with the genus name Prescottella gen. nov. and to replace the illegitimate combination Prescottia equi Jones et al. 2013 with Prescottella equi comb. nov. Antonie Van Leeuwenhoek 103:1405–1407CrossRefPubMedGoogle Scholar
- Kuster E, Williams S (1964) Selection of media for isolation of Streptomycetes. Nat Microbiol 3:928–929Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI—Tech Note 101:1–6Google Scholar
- Taketani RG, Zucchi TD, Melo IS, Mendes R (2013) Whole-genome shotgun sequencing of Rhodococcus erythropolis strain P27, a highly radiation-resistant actinomycete from Antarctica. Genome Announc 1:e00763Google Scholar
- Zhi XY, Li WJ, Stackebrandt E (2009) An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evolut Microbiol 59:589–608CrossRefGoogle Scholar
- Zopf W (1981) Uber Ausscheidung von Fettfarbstoffen (Lipochromen) seitens gewisser Spaltpilze. Berichte der Deutschen Botanischen Gesellschaft 9:22–28Google Scholar