Differences in bacterial composition between men’s and women’s restrooms and other common areas within a public building

  • Priscila Caroline Thiago Dobbler
  • Álvaro Macedo Laureano
  • Deise Schroder Sarzi
  • Ehidy Rocio Peña Cañón
  • Geferson Fernando Metz
  • Anderson Santos de Freitas
  • Beatriz Midori Takagaki
  • Cristiane Barbosa D´Oliveira
  • Victor Satler Pylro
  • André Carlos Copetti
  • Filipe Victoria
  • Marc Redmile-Gordon
  • Daniel Kumazawa Morais
  • Luiz Fernando Wurdig Roesch
Original Paper

Abstract

Humans distribute a wide range of microorganisms around building interiors, and some of these are potentially pathogenic. Recent research established that humans are the main drivers of the indoor microbiome and up to now significant literature has been produced about this topic. Here we analyzed differences in bacterial composition between men’s and women’s restrooms and other common areas within the same public building. Bacterial DNA samples were collected from restrooms and halls of a three-floor building from the Federal University of Pampa, RS, Brazil. The bacterial community was characterized by amplification of the V4 region of the 16S rRNA gene and sequencing. Throughout all samples, the most abundant phylum was Proteobacteria, followed by Actinobacteria, Bacteroidetes and Firmicutes. Beta diversity metrics showed that the structure of the bacterial communities were different among the areas and floors tested, however, only 6–9% of the variation in bacterial communities was explained by the area and floors sampled. A few microorganisms showed significantly differential abundance between men’s and women’s restrooms, but in general, the bacterial communities from both places were very similar. Finally, significant differences among the microbial community profile from different floors were reported, suggesting that the type of use and occupant demographic within the building may directly influence bacterial dispersion and establishment.

Keywords

16S rRNA Microbiome Bacterial diversity Building Dust Indoor microbiome 

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  • Priscila Caroline Thiago Dobbler
    • 1
  • Álvaro Macedo Laureano
    • 1
  • Deise Schroder Sarzi
    • 1
  • Ehidy Rocio Peña Cañón
    • 1
  • Geferson Fernando Metz
    • 1
  • Anderson Santos de Freitas
    • 1
  • Beatriz Midori Takagaki
    • 1
  • Cristiane Barbosa D´Oliveira
    • 1
  • Victor Satler Pylro
    • 2
  • André Carlos Copetti
    • 1
  • Filipe Victoria
    • 1
  • Marc Redmile-Gordon
    • 3
  • Daniel Kumazawa Morais
    • 4
  • Luiz Fernando Wurdig Roesch
    • 1
  1. 1.Centro Interdisciplinar de Pesquisas em BiotecnologiaUniversidade Federal do Pampa (UNIPAMPA)São GabrielBrazil
  2. 2.Soil Microbiology Laboratory, Department of Soil Science, Luiz de Queiroz” College of AgricultureUniversity of São Paulo – ESALQ/USPPiracicabaBrazil
  3. 3.Department of Sustainable Soils and Grassland SystemsRothamsted ResearchHarpendenUK
  4. 4.Biosystems Informatics and Genomics GroupRené Rachou Research CenterBelo HorizonteBrazil

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