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Antonie van Leeuwenhoek

, Volume 111, Issue 4, pp 525–531 | Cite as

Spathaspora piracicabensis f. a., sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil

  • Camila S. Varize
  • Raquel M. Cadete
  • Lucas D. Lopes
  • Renata M. Christofoleti-Furlan
  • Marc-André Lachance
  • Carlos A. Rosa
  • Luiz C. BassoEmail author
Original Paper

Abstract

Two strains of a novel yeast species were isolated from rotting wood of an ornamental tree (purple quaresmeira, Tibouchina granulosa, Melastomataceae) in an Atlantic Rainforest area in Brazil. Analysis of the sequences of the internal transcribed spacer (ITS-5.8S) region and the D1/D2 domains of the large subunit rRNA gene showed that this species belongs to the Spathaspora clade, and is phylogenetically related to Spathaspora brasiliensis, Candida materiae and Sp. girioi. The novel species ferments D-xylose, producing ethanol, with amounts between 3.37 and 3.48 g L−1 ethanol from 2% d-xylose. Ascospores were not observed from this new species. The name Spathaspora piracicabensis f. a., sp. nov. is proposed to accommodate these isolates. The type strain is UFMG-CM-Y5867T (= CBS 15054T = ESALQ-I54T). The MycoBank number is MB 822,320.

Keywords

Atlantic Rainforest Novel yeast species Rotting wood Spathaspora d-xylose-fermenting yeast 

Notes

Funding

This work was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—Brazil, Grant Numbers 457,499/2014-1 and 160143/2014-4), and Fundação do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, Grant Number APQ-01525-14).

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Supplementary material

10482_2017_974_MOESM1_ESM.pdf (88 kb)
Phylogenetic placement of Spathaspora piracicabensis sp. nov. based on ITS-5.8S region sequences. The tree was constructed by Maximum Likelihood analysis of 379 aligned positions using the Tamura-Nei substitution model with a gamma rate distribution and invariant sites. Bootstraps were determined from 1000 pseudoreplicates and values above 50% are indicated in the tree. Bar, 0.1 substitutions per site. Supplementary material 1 (PDF 87 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  • Camila S. Varize
    • 1
  • Raquel M. Cadete
    • 2
  • Lucas D. Lopes
    • 1
  • Renata M. Christofoleti-Furlan
    • 1
  • Marc-André Lachance
    • 3
  • Carlos A. Rosa
    • 2
  • Luiz C. Basso
    • 1
    Email author
  1. 1.Departamento de Ciências Biológicas, Escola Superior de Agricultura Luiz de QueirozUniversidade de São PauloPiracicabaBrazil
  2. 2.Departamento de MicrobiologiaUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  3. 3.Department of BiologyUniversity of Western OntarioLondonCanada

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