Spathaspora piracicabensis f. a., sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil
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Two strains of a novel yeast species were isolated from rotting wood of an ornamental tree (purple quaresmeira, Tibouchina granulosa, Melastomataceae) in an Atlantic Rainforest area in Brazil. Analysis of the sequences of the internal transcribed spacer (ITS-5.8S) region and the D1/D2 domains of the large subunit rRNA gene showed that this species belongs to the Spathaspora clade, and is phylogenetically related to Spathaspora brasiliensis, Candida materiae and Sp. girioi. The novel species ferments D-xylose, producing ethanol, with amounts between 3.37 and 3.48 g L−1 ethanol from 2% d-xylose. Ascospores were not observed from this new species. The name Spathaspora piracicabensis f. a., sp. nov. is proposed to accommodate these isolates. The type strain is UFMG-CM-Y5867T (= CBS 15054T = ESALQ-I54T). The MycoBank number is MB 822,320.
KeywordsAtlantic Rainforest Novel yeast species Rotting wood Spathaspora d-xylose-fermenting yeast
This work was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—Brazil, Grant Numbers 457,499/2014-1 and 160143/2014-4), and Fundação do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, Grant Number APQ-01525-14).
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Conflict of interest
The authors declare no conflicts of interest.
- Cadete RM, Melo MA, Zilli JE, Vital MJ, Mouro A, Prompt AH, Gomes FCO, Stambuk BU, Lachance MA, Rosa CA (2013) Spathaspora brasiliensis sp. nov., Spathaspora suhii sp. nov., Spathaspora roraimanensis sp. nov. and Spathaspora xylofermentans sp. nov., four novel d-xylose-fermenting yeast species from Brazilian Amazonian forest. Antonie Van Leeuwenhoek 103:421–431CrossRefPubMedGoogle Scholar
- Cadete RM, Melo-Cheab MA, Dussán KJ, Rodrigues RCLB, da Silva SS, Gomes FCO, Rosa CA (2017) Production of bioethanol in sugarcane bagasse hemicellulosic hydrolysate by Scheffersomyces parashehatae, Scheffersomyces illinoinensis, and Spathaspora arborariae isolated from Brazilian ecosystems. J Appl Microbiol. https://doi.org/10.1111/jam.13559 PubMedGoogle Scholar
- Lachance MA (2012) In defense of yeast sexual life cycles: the forma asexualis: an informal proposal. Yeast Newsletter 61:24–25Google Scholar
- Lopes MR, Morais CG, Kominek J, Cadete RM, Soares MA, Uetanabaro APT, Fonseca C, Lachance MA, Hittinger CT, Rosa CA (2016) Genomic analysis and d-xylose fermentation of three novel Spathaspora species: Spathaspora girioi sp. nov., Spathaspora hagerdaliae fa, sp. nov. and Spathaspora gorwiae fa, sp. nov. FEMS Yeast Res 16:1–12CrossRefGoogle Scholar
- Morais CG, Batista TM, Kominek J, Borelli BM, Furtado C, Moreira RG, Franco GR, Rosa LH, Fonseca C, Hittinger CT, Lachance MA, Rosa CA (2017) Spathaspora boniae sp. nov., a d-xylose-fermenting species in the Candida albicans-Lodderomyces clade. Int J Syst Evol Microbiol 67:3798–3805CrossRefPubMedGoogle Scholar
- O’Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds) The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematic. CAB International, Wallingford, pp 225–233Google Scholar
- White TJ, Bruns T, Lee SJWT, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc 18:315–322Google Scholar