Haematospirillum and insect Wolbachia DNA in avian blood

  • Sándor Hornok
  • Nóra Ágh
  • Nóra Takács
  • Jenő Kontschán
  • Regina Hofmann-Lehmann
Short Communication

Abstract

In this study, blood samples of 259 Acrocephalus sp. warblers were molecularly analysed for Anaplasmataceae and Rhodospirillaceae based on PCR amplification of 16S rRNA gene fragments. One bird blood sample (from Reed Warbler, Acrocephalus scirpaceus) yielded a sequence with 99.8% identity to Haematospirillum jordaniae. This is the first molecular evidence for the occurrence of this species in the blood of any vertebrate other than human. Another bird blood sample (from Marsh Warbler: Acrocephalus palustris) yielded a Wolbachia sequence, closely related to a moth endosymbiont with 99.8% identity. A nematode origin of Wolbachia DNA detected here in avian blood can be excluded, because results of phylogenetic analysis showed its closest alignment with insect wolbachiae. This is the first finding of insect Wolbachia DNA in the circulatory system of birds, which can be explained either by the inoculation of wolbachiae by blood-sucking vectors, or passing of Wolbachia DNA from the gut into the blood of this insectivorous bird species.

Keywords

Anaplasmataceae Aves Rhodospirillaceae 16S rRNA gene 

Notes

Funding

This study was funded by OTKA (Grant Numbers 115854 and 108571).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Research was permitted by Middle-Danube-Valley Inspectorate for Environmental Protection, Nature Conservation and Water Management (under Registration Number KTF: 27251-1/2014). Blood sampling was performed by Nóra Ágh (Certificate Registration Number 6/2015, issued by the Institutional Animal Welfare Committee).

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Department of Parasitology and ZoologyUniversity of Veterinary MedicineBudapestHungary
  2. 2.Department of Biomathematics and InformaticsUniversity of Veterinary MedicineBudapestHungary
  3. 3.Plant Protection Institute, Centre for Agricultural ResearchHungarian Academy of SciencesBudapestHungary
  4. 4.Vetsuisse Faculty, Clinical Laboratory and Center for Clinical StudiesUniversity of ZürichZurichSwitzerland

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