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Antonie van Leeuwenhoek

, Volume 111, Issue 2, pp 209–226 | Cite as

Biodiversity and ecology of flower-associated actinomycetes in different flowering stages of Protea repens

  • Zander R. Human
  • Casparus J. Crous
  • Francois Roets
  • Stephanus N. Venter
  • Michael J. Wingfield
  • Z. Wilhelm de Beer
Original Paper

Abstract

Actinomycete bacteria have previously been reported from reproductive structures (infructescences) of Protea (sugarbush/suikerbos) species, a niche dominated by fungi in the genera Knoxdaviesia and Sporothrix. It is probable that these taxa have symbiotic interactions, but a lack of knowledge regarding their diversity and general ecology precludes their study. We determined the diversity of actinomycetes within Protea repens inflorescence buds, open inflorescences, young and mature infructescences, and leaf litter surrounding these trees. Since the P. repens habitat is fire-prone, we also considered the potential of these bacteria to recolonise infructescences after fire. Actinomycetes were largely absent from flower buds and inflorescences but were consistently present in young and mature infructescences. Two Streptomyces spp. were the most consistent taxa recovered, one of which was also routinely isolated from leaf litter. Lower colonisation rates were evident in samples from a recently burnt site. One of the most consistent taxa isolated from older trees in the unburnt site was absent from this site. Our findings show that P. repens has a distinct community of actinomycetes dominated by a few species. These communities change over time and infructescence developmental stage, season and the age of the host population. Mature infructescences appear to be important sources of inoculum for some of the actinomycetes, seemingly disrupted by fire. Increased fire frequency limiting maturation of P. repens infructescences could thus impact future actinomycete colonisation in the landscape. Streptomyces spp. are likely to share this niche with the ophiostomatoid fungi, which merits further study regarding their interactions and mode of transfer.

Keywords

Proteaceae Streptomyces Cape Floristic Kingdom Sporothrix Knoxdaviesia 

Notes

Acknowledgements

We thank the National Research Foundation (NRF) and the Department of Science and Technology (DST)/NRF Centre for Tree Health Biotechnology (CTHB) for financial support. We also thank the Cape Nature Conservation Board for providing collection permits. We further wish to thank Glenda Brits from the Department of Education Innovation, University of Pretoria, for producing Fig. 3.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2017_942_MOESM1_ESM.tif (1.2 mb)
Supplementary Figure S1 Regression coefficients (β), odds ratios (OR) and p-values from binary logistic regression of presence or absence of all actinomycetes, OTU1 and OTU2 in infructescences (TIFF 1244 kb)
10482_2017_942_MOESM2_ESM.docx (3.5 mb)
Supplementary Table S1 Species richness estimators and colonisation frequencies for all sample categories in this study. Table S2 Results of mixed effects models testing the effect of inflorescence organ on species richness and presence of actinomycetes. Supplementary Methods Actinomycete colonisation frequency and species richness on different P. repens flower organs (DOCX 3564 kb)

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Authors and Affiliations

  1. 1.Department of Microbiology and Plant Pathology, Forestry and Agriculture Biotechnology InstituteUniversity of PretoriaPretoriaSouth Africa
  2. 2.Department of Plant and Soil Sciences, Forestry and Agriculture Biotechnology InstituteUniversity of PretoriaPretoriaSouth Africa
  3. 3.Faculdade de Ciências, Centre for Ecology, Evolution and Environmental Changes (cE3c)Universidade de LisboaLisbonPortugal
  4. 4.Department of Conservation Ecology and EntomologyStellenbosch UniversityStellenboschSouth Africa

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