Polyamine-binding protein PotD2 is required for stress tolerance and virulence in Actinobacillus pleuropneumoniae
- 249 Downloads
Actinobacillus pleuropneumoniae is the cause of porcine contagious pleuropneumonia, which is one of the most important respiratory diseases in swine and causes huge economic losses in the swine industry. PotD, a polyamine-binding protein, has been well characterised in many pathogens of humans and animals. In this study, a ΔpotD2 mutant of A. pleuropneumoniae strain MS71 (serovar 1) was constructed successfully by homologous recombination. Growth curves of different strains showed that the growth of the ΔpotD2 mutant was affected greatly in the logarithmic phase compared with that of parental strain. In vitro stress assays revealed that the viability of ΔpotD2 mutant strain was significantly impaired under multiple environmental stresses, including high temperature, oxidation and hyperosmosis. Additionally, the ΔpotD2 mutant caused significantly decreased mortality in a mouse model. Taken together, the findings in this study suggest an important role of PotD2 in the growth, stress tolerance and virulence of A. pleuropneumoniae.
KeywordsActinobacillus pleuropneumoniae potD2 gene Stress tolerance Virulence
This research was financially supported by a grant from the Special Fund for Agro-Scientific Research in the Public Interest (No. 201303034) and a grant from the Special Fund for Sichuan Science and Technology Support Program (No. 2013NZ0056).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The animal experiments were conducted in strict accordance with the recommendations in the China Regulations for the Administration of Affairs Concerning Experimental Animals (1988) and had been approved by the Institutional Animal Care and Use Committee of Sichuan Agricultural University (Approval Number BK2014-047), Sichuan, China.
- Zhang F, Cao S, Zhu Z, Yang Y, Wen X, Chang YF, Huang X, Wu R, Wen Y, Yan Q (2016a) Immunoprotective efficacy of six in vivo-induced antigens against Actinobacillus pleuropneumoniae as potential vaccine candidates in murine model. Front Microbiol 38:728–732Google Scholar