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Antonie van Leeuwenhoek

, Volume 110, Issue 12, pp 1537–1553 | Cite as

Two new Leptographium spp. reveal an emerging complex of hardwood-infecting species in the Ophiostomatales

  • Robert JankowiakEmail author
  • Beata Strzałka
  • Piotr Bilański
  • Riikka Linnakoski
  • Truls Aas
  • Halvor Solheim
  • Małgorzata Groszek
  • Z. Wilhelm de Beer
Original Paper

Abstract

Species of Leptographium are generally characterized by mononematous conidiophores and are commonly associated with bark beetles and weevils. These species are responsible for sapstain and in some cases serious diseases on a range of primarily coniferous trees. In comparison with coniferous trees, the occurrence of Leptographium species on hardwood trees has been poorly studied in Europe. During a survey of ophiostomatoid fungi on various tree species in Norway and Poland, three unusual species, which fit the broader morphological description of Leptographium spp., were found in association with Scolytus ratzeburgi, Dryocoetes alni and Trypodendron domesticum on a variety of hardwoods, and from wounds on Tilia cordata. Phylogenetic analyses of sequence data for three gene regions (ITS2-LSU, β-tubulin, and TEF1-α) showed that these Leptographium species are phylogenetically closely related to each other and form a well-supported lineage that included Grosmannia grandifoliae and Leptographium pruni. The first species could be distinguished from the other Leptographium species based on conidiophores arising from spiral hyphae, chlamydospore-like structures and a hyalorhinocladiella-like synanamorph in culture. The second species differs from the previous one by having distinctly shorter conidiophores and smaller conidia. This species also produces a well-developed sporothrix-like synanamorph with denticulate conidiogenous cells. Based on these unusual morphological characteristics and distinct DNA sequences, these fungi were recognised as new taxa for which the names Leptographium trypodendri sp. nov. and L. betulae sp. nov. are provided. The third group of isolates belonged to Grosmannia grandifoliae, representing the first report of this species outside of the USA. The newly defined G. grandifoliae complex is the first species complex in Leptographium s.l. consisting of only hardwood-infecting species.

Keywords

Bark beetle-associated fungi Hardwoods Ophiostomatoid fungi Leptographium trypodendri Leptographium betulae 

Notes

Acknowledgements

This work was supported by the National Science Centre, Poland (contract No. UMO-2014/15/B/NZ9/00560). The work in Norway is partly financed by Norwegian biodiversity information centre and is part of a Master thesis for T.A.

Conflict of interest

The authors declare that have no conflict of interest.

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Robert Jankowiak
    • 1
  • Beata Strzałka
    • 1
  • Piotr Bilański
    • 2
  • Riikka Linnakoski
    • 3
    • 4
  • Truls Aas
    • 5
  • Halvor Solheim
    • 5
    • 6
  • Małgorzata Groszek
    • 1
  • Z. Wilhelm de Beer
    • 4
  1. 1.Department of Forest Pathology, Mycology and Tree Physiology, Institute of Forest Ecosystem ProtectionUniversity of Agriculture in KrakowKrakowPoland
  2. 2.Department of Forest Protection, Entomology and Forest Climatology, Institute of Forest Ecosystem ProtectionUniversity of Agriculture in KrakowKrakowPoland
  3. 3.Department of Forest SciencesUniversity of HelsinkiHelsinkiFinland
  4. 4.Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  5. 5.Department of Ecology and Natural Resource ManagementNorwegian University of Life SciencesÅsNorway
  6. 6.Norwegian Institute of Bioeconomy ResearchÅsNorway

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