Denitrifying bacterial communities display different temporal fluctuation patterns across Dutch agricultural soils

  • Nguyen E. López-Lozano
  • Michele C. Pereira e Silva
  • Franck Poly
  • Nadine Guillaumaud
  • Jan Dirk van Elsas
  • Joana Falcão Salles
Original Paper

DOI: 10.1007/s10482-017-0898-3

Cite this article as:
López-Lozano, N.E., Pereira e Silva, M.C., Poly, F. et al. Antonie van Leeuwenhoek (2017). doi:10.1007/s10482-017-0898-3


Considering the great agronomic and environmental importance of denitrification, the aim of the present study was to study the temporal and spatial factors controlling the abundance and activity of denitrifying bacterial communities in a range of eight agricultural soils over 2 years. Abundance was quantified by qPCR of the nirS, nirK and nosZ genes, and the potential denitrification enzyme activity (DEA) was estimated. Our data showed a significant temporal variation considerably high for the abundance of nirK-harboring communities, followed by nosZ and nirS communities. Regarding soil parameters, the abundances of nosZ, nirS and nirK were mostly influenced by organic material, pH, and slightly by NO3, respectively. Soil texture was the most important factor regulating DEA, which could not be explained by the abundance of denitrifiers. Analyses of general patterns across lands to understand the soil functioning is not an easy task because the multiple factors influencing processes such as denitrification can skew the data. Careful analysis of atypical sites are necessary to classify the soils according to trait similarity and in this way reach a better predictability of the denitrifiers dynamics.


Gene abundance nosnirnirPotential denitrification Soil texture 

Supplementary material

10482_2017_898_MOESM1_ESM.docx (61 kb)
Supplementary material 1 (DOCX 60 kb)
10482_2017_898_MOESM2_ESM.docx (1.2 mb)
Supplementary material 2 (DOCX 1223 kb)

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Department of Microbial Ecology, Centre for Life SciencesUniversity of GroningenGroningenThe Netherlands
  2. 2.División de Ciencias AmbientalesInstituto Potosino de Investigación Científica y Tecnológica (IPICyT)San Luis PotosíMexico
  3. 3.Microbial Ecology Centre (UMR 5557 CNRS-Université Lyon 1; USC 1193 INRA)VilleurbanneFrance
  4. 4.Soil Microbiology Laboratory, Soil Science Department, College of Agriculture “Luiz de Queiroz”University of Sao PauloSão PauloBrazil

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