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Antonie van Leeuwenhoek

, Volume 110, Issue 8, pp 1027–1034 | Cite as

Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample

  • Jie Zhu
  • Hui-Min Wang
  • Qi Zhang
  • Wei-Wei Dong
  • De-Long Kong
  • Yan-Wei Wang
  • Jin-Long Song
  • Xu Jiang
  • Shu-Miao Zhao
  • Wei ZhangEmail author
  • Zhi-Yong RuanEmail author
Original Paper

Abstract

A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11T, was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11T were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11T was found to be able to grow at 4–40 °C (optimum 15 °C), pH 7.5–2.0 (optimum 9.5) and 0–2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11T is closely related to Arenimonas metalli CF5-1T (98.0%), Arenimonas aquaticum NA-09T (97.9%), Arenimonas donghaensis HO3-R19T (95.6%) and Arenimonas aestuarii S2-21T (95.3%). The DNA–DNA hybridization values between the isolate and A. metalli CGMCC 1.10787T, A. aquaticum KACC 14663T, A. donghaensis KACC 11381T were 41.0 ± 1.7, 44.7 ± 1.4 and 42.8 ± 1.2%, respectively. The genomic DNA G+C content was found to be 66.5 mol% as determined by the T m method. The major cellular fatty acids were identified as iso-C15:0 and iso-C16:0. The major isoprenoid quinone was identified as ubiquinone 8 (Q-8). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two phospholipids and five unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM-WHM-D11T is concluded to represent a novel species within the genus Arenimonas, for which the name Arenimonas alkanexedens sp. nov. is proposed. The type strain is LAM-WHM-D11T (ACCC 19750T = JCM 30464T).

Keywords

Arenimonas alkanexedens sp. nov. Frozen soil sample 16S rRNA gene Polyphasic taxonomy 

Notes

Acknowledgements

This work was supported by Open Funds of Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin (Tarim University, BRZD1603), Foundation of the Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China) (No. 2015002) and National Infrastructure of Microbial Resources (NIMR-2017-1).

Supplementary material

10482_2017_876_MOESM1_ESM.pdf (335 kb)
Supplementary material 1 (PDF 335 kb)

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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  • Jie Zhu
    • 1
    • 2
  • Hui-Min Wang
    • 2
  • Qi Zhang
    • 1
  • Wei-Wei Dong
    • 3
  • De-Long Kong
    • 2
  • Yan-Wei Wang
    • 4
  • Jin-Long Song
    • 5
  • Xu Jiang
    • 2
  • Shu-Miao Zhao
    • 3
  • Wei Zhang
    • 1
    Email author
  • Zhi-Yong Ruan
    • 2
    Email author
  1. 1.College of Life ScienceXinjiang Normal UniversityUrumqiChina
  2. 2.Key Laboratory of Microbial Resources (Ministry of Agriculture, China)Institute of Agricultural Resources and Regional Planning, CAASBeijingPeople’s Republic of China
  3. 3.State Key Laboratory of Agricultural Microbiology, College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  4. 4.Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China)Biogas Institute of Ministry of AgricultureChengduChina
  5. 5.Chinese Academy of Fishery SciencesBeijingChina

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