Characterization of Marinovum faecis sp. nov., an alphaproteobacterium isolated from marine sediment
A Gram-negative, strictly aerobic, chemoheterotrophic, beige-pigmented, ovoid bacterium, designated YP194T, was isolated from marine sediment in the Republic of Korea. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain belongs to the family Rhodobacteraceae of the class Alphaproteobacteria, with high sequence similarity (98.4%) to Marinovum algicola FF3T. The DNA–DNA relatedness values between strains YP194T and M. algicola FF3T were 34.1 ± 2.7%. The DNA G+C content of strain YP194T was 63.1 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acid was C18:1 ω7c (77.6%). Strain YP194T produced phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids as polar lipids. From the combination of genotypic and phenotypic characteristics and the distinct phylogenetic position, the strain is considered to represent a novel species of the genus Marinovum for which the name Marinovum faecis sp. nov. is proposed. The type strain of M. faecis sp. nov. is YP194T (= KCCM 90263T = NBRC 111905T).
KeywordsAlphaproteobacteria Marinovum faecis sp. nov. 16S rRNA gene Polyphasic taxonomy Marine sediment
This work was supported by the National Research Foundation of Korea Grants (NRF-2014R1A1A2057302), Korea.
Conflicts of interest
The authors declare that they have no conflict of interest.
Research involving human participants and/or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
- Garrity GM, Bell JA, Lilburn T (2005) Family I. Rhodobacteraceae fam. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 2C, 2nd edn. Springer, New York, p 161Google Scholar
- Iwaki H, Yasukawa N, Fujioka M, Takada K, Hasegawa Y (2013) Isolation and characterization of a marine cyclohexylacetate-degrading bacterium Lutimaribacter litoralis sp. nov., and reclassification of Oceanicola pacificus as Lutimaribacter pacificus comb. nov. Curr Microbiol 66:588–593CrossRefPubMedGoogle Scholar
- Martens T, Heidorn T, Pukall R, Simon M, Tindall BJ, Brinkhoff T et al (2006) Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 56:1293–1304CrossRefPubMedGoogle Scholar
- Power DA, Johnson JA (2009) Difco™ and BBL™ Manual: manual of microbiological culture media, 2nd edn. Becton Dickinson and Company, Sparks, pp 359–360Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, NewarkGoogle Scholar
- Uchino Y, Hirata A, Yokota A, Sugiyama J (1998) Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44:201–210CrossRefPubMedGoogle Scholar