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Antonie van Leeuwenhoek

, Volume 110, Issue 7, pp 963–969 | Cite as

Characterization of Marinovum faecis sp. nov., an alphaproteobacterium isolated from marine sediment

  • Jaewoo Yoon
  • So Young Kim
  • Songhee Park
  • Hyukjae ChoiEmail author
Original Paper

Abstract

A Gram-negative, strictly aerobic, chemoheterotrophic, beige-pigmented, ovoid bacterium, designated YP194T, was isolated from marine sediment in the Republic of Korea. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the novel marine strain belongs to the family Rhodobacteraceae of the class Alphaproteobacteria, with high sequence similarity (98.4%) to Marinovum algicola FF3T. The DNA–DNA relatedness values between strains YP194T and M. algicola FF3T were 34.1 ± 2.7%. The DNA G+C content of strain YP194T was 63.1 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acid was C18:1 ω7c (77.6%). Strain YP194T produced phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids as polar lipids. From the combination of genotypic and phenotypic characteristics and the distinct phylogenetic position, the strain is considered to represent a novel species of the genus Marinovum for which the name Marinovum faecis sp. nov. is proposed. The type strain of M. faecis sp. nov. is YP194T (= KCCM 90263T = NBRC 111905T).

Keywords

Alphaproteobacteria Marinovum faecis sp. nov. 16S rRNA gene Polyphasic taxonomy Marine sediment 

Notes

Acknowledgements

This work was supported by the National Research Foundation of Korea Grants (NRF-2014R1A1A2057302), Korea.

Conflicts of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2017_867_MOESM1_ESM.pdf (57 kb)
Thin-layer chromatograms showing the total polar lipid compositions of YP194T. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid, molybdenum blue, α-naphthol, ninhydrin and Dragendorff’s reagent. PG: phosphatidylglycerol, PE: phosphatidylenthanolamine, PC: phosphatidylcholine, UAL: unidentified aminolipid, UPL: unidentified phospholipid, UL: unidentified lipid Supplementary material 1 (PDF 57 kb)
10482_2017_867_MOESM2_ESM.docx (12 kb)
Supplementary material 2 (DOCX 11 kb)

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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  1. 1.College of PharmacyKeimyung UniversityDaeguRepublic of Korea
  2. 2.College of PharmacyYeungnam UniversityGyeongsanRepublic of Korea

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