Antonie van Leeuwenhoek

, Volume 110, Issue 6, pp 727–736 | Cite as

Reassessment of the taxonomic position of Burkholderia andropogonis and description of Robbsia andropogonis gen. nov., comb. nov.

  • Lucilene Lopes-Santos
  • Daniel Bedo Assumpção Castro
  • Mariana Ferreira-Tonin
  • Daniele Bussioli Alves Corrêa
  • Bevan Simon Weir
  • Duckchul Park
  • Laura Maria Mariscal Ottoboni
  • Júlio Rodrigues Neto
  • Suzete Aparecida Lanza DestéfanoEmail author
Original Paper


The phylogenetic classification of the species Burkholderia andropogonis within the Burkholderia genus was reassessed using 16S rRNA gene phylogenetic analysis and multilocus sequence analysis (MLSA). Both phylogenetic trees revealed two main groups, named A and B, strongly supported by high bootstrap values (100%). Group A encompassed all of the Burkholderia species complex, whi.le Group B only comprised B. andropogonis species, with low percentage similarities with other species of the genus, from 92 to 95% for 16S rRNA gene sequences and 83% for conserved gene sequences. Average nucleotide identity (ANI), tetranucleotide signature frequency, and percentage of conserved proteins POCP analyses were also carried out, and in the three analyses B. andropogonis showed lower values when compared to the other Burkholderia species complex, near 71% for ANI, from 0.484 to 0.724 for tetranucleotide signature frequency, and around 50% for POCP, reinforcing the distance observed in the phylogenetic analyses. Our findings provide an important insight into the taxonomy of B. andropogonis. It is clear from the results that this bacterial species exhibits genotypic differences and represents a new genus described herein as Robbsia andropogonis gen. nov., comb. nov.


Bacterial disease Genome sequence MLSA 16S rRNA gene 



We thank the São Paulo Research Foundation (FAPESP) for financial support (Grant #2011/12222-2 and #2011/50813-2).

Supplementary material

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Supplementary material 1 (XLSX 13 kb)
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Supplementary material 2 (XLSX 49 kb)
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Supplementary material 3 (DOCX 62 kb)


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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  • Lucilene Lopes-Santos
    • 1
  • Daniel Bedo Assumpção Castro
    • 3
  • Mariana Ferreira-Tonin
    • 1
  • Daniele Bussioli Alves Corrêa
    • 1
  • Bevan Simon Weir
    • 2
  • Duckchul Park
    • 2
  • Laura Maria Mariscal Ottoboni
    • 3
  • Júlio Rodrigues Neto
    • 1
  • Suzete Aparecida Lanza Destéfano
    • 1
    Email author
  1. 1.Laboratório de Bacteriologia VegetalInstituto BiológicoCampinasBrazil
  2. 2.Landcare ResearchAucklandNew Zealand
  3. 3.Centro de Biologia Molecular e Engenharia GenéticaUniversidade Estadual de Campinas - UNICAMPCampinasBrazil

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