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Antonie van Leeuwenhoek

, Volume 110, Issue 4, pp 553–562 | Cite as

Enterovirga rhinocerotis gen. nov., sp. nov., isolated from Rhinoceros unicornis faeces

  • Xiu Chen
  • Qin-Yuan Li
  • Gui-Ding Li
  • Hui Lei
  • Yi JiangEmail author
  • Li HanEmail author
  • Xue-Shi Huang
  • Cheng-Lin Jiang
Original Paper

Abstract

A novel strain, YIM 100770T, was isolated from Rhinoceros unicornis faeces collected from Yunnan Wild Animal Park, China. The taxonomic status was determined based on the physiological, biochemical and phylogenetic characteristics. Strain YIM 100770T was observed to be rod-shaped, non-motile, Gram-stain negative and aerobic. The G+C content of the genomic DNA was determined to be 68.5 mol%. The cells of strain YIM 100770T contain ubiquinone Q-10 as the respiratory quinone. The major fatty acids (>1%) were identified as Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c; 78.1%), Summed feature 4 (iso-C17:1-I and/or anteiso-C17:1-B; 12.9%), C19:0 cyclo ω8c (2.8%), C16:0 (2.2%) and C18:0 (2.2%). Comparison of 16S rRNA gene sequences revealed the strain show high similarities with the members of the genera Psychroglaciecola (94.5%), Methylobacterium (90.5–94.1%) and Microvirga (92.0–93.3%) in the family Methylobacteriaceae. In addition, the strain also showed high similarities with the members of the genera Chelatococcus (93.7–94.0%) and Pseudochelatococcus (93.1–93.7%) in the family Beijerinckiacea, and the genus Bosea (93.1–93.8%) in the family Bradyrhizobiaceae. The phylogenetic analysis, combined with the chemical characteristics, suggest that the strain represents a novel genus in the order Rhizobiales of the class Alphaproteobacteria, for which the name Enterovirga rhinocerotis gen. nov., sp. nov. is proposed. The type strain of E. rhinocerotis is YIM 100770T (=DSM 25903T = CCTCC AB 2012048T).

Keywords

Enterovirga rhinocerotis gen. nov., sp. nov. 16S rRNA gene Faeces 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (Nos. 31270001, 31460005 and 81573327), and supported by Program for New Century Excellent Talents in University and Yunnan Provincial Society Development Project (2014BC006). Thanks for the assistance of Yunnan Wild Animal Park, and thanks for the assistance from Mr. Jiang Liu and You-Long Li.

Supplementary material

10482_2016_823_MOESM1_ESM.docx (3.1 mb)
Supplementary material 1 (DOCX 3196 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Xiu Chen
    • 1
    • 2
  • Qin-Yuan Li
    • 1
  • Gui-Ding Li
    • 1
    • 2
  • Hui Lei
    • 2
  • Yi Jiang
    • 1
    Email author
  • Li Han
    • 2
    Email author
  • Xue-Shi Huang
    • 2
  • Cheng-Lin Jiang
    • 1
  1. 1.Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China
  2. 2.Institute of Microbial Pharmaceuticals, College of Life and Health SciencesNortheastern UniversityShenyangPeople’s Republic of China

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