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Antonie van Leeuwenhoek

, Volume 110, Issue 2, pp 253–270 | Cite as

Characterisation of Pseudomonas spp. and Ochrobactrum sp. isolated from volcanic soil

  • Shashank Kumar Mishra
  • Mohammad Haneef Khan
  • Sankalp Misra
  • Vijay Kant Dixit
  • Praveen Khare
  • Suchi Srivastava
  • Puneet Singh ChauhanEmail author
Original Paper

Abstract

Soil bacteria may have properties of plant growth promotion but not be sufficiently beneficial for plants under stress conditions. This challenge has led researchers to extend their searches into extreme environments for potential soil bacteria with multiple plant beneficial traits as well as abiotic stress tolerance abilities. In the current study, an attempt was made to evaluate soil bacteria from an extreme environment, volcano soils, based on plant growth promoting and abiotic stress mitigating characteristics. The screening led to the isolation of eight (NBRISH4, NBRISH6, NBRISH10, NBRISH11, NBRISH13, NBRISH14, NBRISH16 and NBRISH26) bacterial isolates capable of withstanding stresses, namely temperature (up to 45 °C), salt (up to 2 M NaCl) and drought (up to 60% Poly Ethylene Glycol 6000) in vitro. Further, the selected isolates were notable for their in vitro temporal performance with regards to survival (in terms of colony count), phosphate solubilisation, biofilm formation, auxin, alginate and exo-polysaccharide production abilities under abiotic stresses i.e. 40 °C temperature; 500 mM NaCl salt and drought (PEG) conditions. In vivo seed treatments of individual selected bacteria to maize plants resulted into significant enhancement in root and shoot length, root and shoot fresh and dry weight and number of leaves per plant. Overall, the plant growth promoting and abiotic stress tolerance ability was most evident for bacterial isolate NBRISH6 which was identified as an Ochrobactrum sp. using 16S rRNA based phylogenetic analysis.

Keywords

Abiotic stress Ochrobactrum sp. Plant growth promotion Pseudomonas spp. Volcano 

Notes

Acknowledgements

The study was conducted using the operating funds of the network project Plant Microbe and Soil Interactions (PMSI) (BSC-0117) funded by Council of Scientific and Industrial Research, New Delhi, India. Authors are thankful to the Director, CSIR-NBRI, Lucknow for providing necessary resources to conduct this study.

Supplementary material

10482_2016_796_MOESM1_ESM.docx (120 kb)
Supplementary material 1 (DOCX 120 kb) Supplementary data associated with this article can be found in Figure S1–S3, and Table S1

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Shashank Kumar Mishra
    • 1
  • Mohammad Haneef Khan
    • 1
  • Sankalp Misra
    • 1
  • Vijay Kant Dixit
    • 1
  • Praveen Khare
    • 1
  • Suchi Srivastava
    • 1
  • Puneet Singh Chauhan
    • 1
    Email author
  1. 1.Division of Plant Microbe InteractionsCSIR-National Botanical Research InstituteLucknowIndia

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