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Antonie van Leeuwenhoek

, Volume 109, Issue 12, pp 1593–1603 | Cite as

Pistricoccus aurantiacus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a shark

  • Zhen-Xing Xu
  • Qi-Yun Liang
  • De-Chen Lu
  • Guan-Jun Chen
  • Zong-Jun Du
Original Paper

Abstract

A novel Gram-stain negative, non-motile, moderately halophilic, facultatively anaerobic and spherical bacterium designated strain SS9T was isolated from the gill homogenate of a shark. Cells of SS9T were observed to be 0.8–1.2 μm in diameter. The strain was found to grow optimally at 33 °C, pH 7.0–8.0 and in the presence of 6.0 % (w/v) NaCl. On the basis of 16S rRNA gene phylogeny, strain SS9T can be affiliated with the family Halomonadaceae and is closely related to Chromohalobacter marismortui NBRC 103155T (95.6 % sequence similarity), Halomonas ilicicola SP8T (95.6 %) and Chromohalobacter salexigens DSM 3043T (95.5 %). Multilocus sequence analysis of strain SS9T using the housekeeping genes 16S rRNA, 23S rRNA, gyrB, rpoD and secA revealed the strain’s distinct phylogenetic position, separate from other known genera of the family Halomonadaceae. Strain SS9T was found to contain ubiquinone-9 (Q-9) as the predominant ubiquinone and C18:1 ω7c, C16:0 and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH) as the major fatty acids. The major polar lipids of strain SS9T were identified as phosphatidylglycerol and phosphatidylethanolamine. The DNA G + C content of strain SS9T was determined to be 60.4 mol%. It is evident from phylogenetic, genotypic, phenotypic and chemotaxonomic results that strain SS9T represents a novel species in a new genus, for which the name Pistricoccus aurantiacus gen. nov., sp. nov. is proposed. The type strain is SS9T (=KCTC 42586T = MCCC 1H00111T).

Keywords

Pistricoccus aurantiacus Moderately halophilic bacterium 16S rRNA gene MLSA 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31370057, 31290231) and 2013 Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299).

Supplementary material

10482_2016_760_MOESM1_ESM.docx (232 kb)
Supplementary material 1 (DOCX 232 kb)

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Zhen-Xing Xu
    • 1
  • Qi-Yun Liang
    • 1
  • De-Chen Lu
    • 1
  • Guan-Jun Chen
    • 1
    • 2
  • Zong-Jun Du
    • 1
    • 2
  1. 1.College of Marine ScienceShandong University at WeihaiWeihaiChina
  2. 2.State Key Laboratory of Microbial TechnologyShandong UniversityJinanChina

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