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Antonie van Leeuwenhoek

, Volume 109, Issue 7, pp 945–956 | Cite as

Pan-genome analysis of Aeromonas hydrophila, Aeromonas veronii and Aeromonas caviae indicates phylogenomic diversity and greater pathogenic potential for Aeromonas hydrophila

  • Sandeep Ghatak
  • Jochen Blom
  • Samir Das
  • Rajkumari Sanjukta
  • Kekungu Puro
  • Michael Mawlong
  • Ingudam Shakuntala
  • Arnab Sen
  • Alexander Goesmann
  • Ashok Kumar
  • S. V. Ngachan
Original Paper

Abstract

Aeromonas species are important pathogens of fishes and aquatic animals capable of infecting humans and other animals via food. Due to the paucity of pan-genomic studies on aeromonads, the present study was undertaken to analyse the pan-genome of three clinically important Aeromonas species (A. hydrophila, A. veronii, A. caviae). Results of pan-genome analysis revealed an open pan-genome for all three species with pan-genome sizes of 9181, 7214 and 6884 genes for A. hydrophila, A. veronii and A. caviae, respectively. Core-genome: pan-genome ratio (RCP) indicated greater genomic diversity for A. hydrophila and interestingly RCP emerged as an effective indicator to gauge genomic diversity which could possibly be extended to other organisms too. Phylogenomic network analysis highlighted the influence of homologous recombination and lateral gene transfer in the evolution of Aeromonas spp. Prediction of virulence factors indicated no significant difference among the three species though analysis of pathogenic potential and acquired antimicrobial resistance genes revealed greater hazards from A. hydrophila. In conclusion, the present study highlighted the usefulness of whole genome analyses to infer evolutionary cues for Aeromonas species which indicated considerable phylogenomic diversity for A. hydrophila and hitherto unknown genomic evidence for pathogenic potential of A. hydrophila compared to A. veronii and A. caviae.

Keywords

Aeromonas Antimicrobial resistance Pan-genome Phylogeny Recombination Virulence 

Notes

Acknowledgments

Authors are thankful to Director, ICAR Research Complex for NEH Region for providing necessary facilities, to Administrator, Nazareth Hospital, Shillong for her support. First author is also thankful to Ms. Dyuti Purkait and Dr. D. Chakraborty for help with data analysis.

Compliance with ethical standards

Conflict of interest

No conflict of interest declared.

Supplementary material

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Sandeep Ghatak
    • 1
  • Jochen Blom
    • 2
  • Samir Das
    • 1
  • Rajkumari Sanjukta
    • 1
  • Kekungu Puro
    • 1
  • Michael Mawlong
    • 3
  • Ingudam Shakuntala
    • 1
  • Arnab Sen
    • 1
  • Alexander Goesmann
    • 2
  • Ashok Kumar
    • 4
  • S. V. Ngachan
    • 5
  1. 1.Division of Animal HealthICAR Research Complex for NEH RegionUmiamIndia
  2. 2.Bioinformatics and Systems BiologyJustus-Liebig-University GiessenGiessenGermany
  3. 3.Nazareth HospitalLaitumukhrah, ShillongIndia
  4. 4.Division of Veterinary Public HealthIndian Veterinary Research InstituteIzatnagarIndia
  5. 5.ICAR Research Complex for NEH RegionUmiamIndia

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