Antonie van Leeuwenhoek

, Volume 109, Issue 4, pp 589–601 | Cite as

Novel ophiostomatalean fungi from galleries of Cyrtogenius africus (Scolytinae) infesting dying Euphorbia ingens

  • Johannes Alwyn van der Linde
  • Diana L. Six
  • Wilhelm Z. De Beer
  • Michael J. Wingfield
  • Jolanda RouxEmail author
Original Paper


Euphorbia ingens trees have been dying in large numbers in the Limpopo Province of South Africa for approximately 15 years. The ambrosia beetle Cyrtogenius africus is often found infesting diseased and dying trees. The aim of this study was to identify the ophiostomatoid fungi occurring in the galleries of C. africus. Logs infested with this beetle were collected from the KwaZulu-Natal, Limpopo, Mpumalanga, and North West Provinces of South Africa. Fungi belonging to the Ophiostomatales were identified based on morphology and comparison of sequence data for the β-tubulin, ITS1-5.8S-ITS2 and LSU gene regions. A novel species of Ophiostoma and a novel genus in the Ophiostomatales were identified. Inoculation studies with these fungi produced lesions in the branches of healthy E. ingens trees.


Ophiostoma Ophiostomataceae Ophiostomatalean fungi Ophiostomatales Scolytinae 



We thank the Department of Science and Technology (DST), National Research Foundation (NRF) and DST/NRF Centre of Excellence in Tree Health Biotechnology (CTHB), for financial support; Dr. Hugh Glen for assistance with Latin names and Dr. Roger Beaver for assistance with beetle identification. Mr. Gert van der Merwe, Mr. Thomas Ndala, Mr. Henk Fourie and Buyskop Game Farm are thanked for permission to use their properties for field studies.


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Johannes Alwyn van der Linde
    • 1
  • Diana L. Six
    • 2
  • Wilhelm Z. De Beer
    • 1
  • Michael J. Wingfield
    • 1
  • Jolanda Roux
    • 1
    Email author
  1. 1.Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.College of Forestry and Conservation, Department of Ecosystem and Conservation SciencesThe University of MontanaMissoulaUSA

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