Taxonomic characterisation of Proteus terrae sp. nov., a N2O-producing, nitrate-ammonifying soil bacterium
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In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N2O. The amount of nitrous oxide produced was influenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus, showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNA–DNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687T =DSM 29910T =LMG 28659T) is proposed.
KeywordsProteus terrae sp. nov. Phylogenetic characterization Phenotypic characterisation Dissimilatory nitrate reduction to ammonium Nitrous oxide production C/N ratio Fen peat soil
We wish to thank Mrs. B. Selch, Mrs. S. Weinert, Mr. B. Gusovius (ZALF-Müncheberg), Mrs. A. Wasner, Mrs. B. Sträubler and Mrs. G. Pötter (DSMZ-Braunschweig), and Mr. Th. Rossoll (IGB-Berlin) for their excellent technical assistance.
- Behrendt U, Schumann P, Stieglmeier M, Pukall R, Augustin J, Spröer C, Schwendner P, Moissl-Eichinger C, Ulrich A (2010) Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity - description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room. Syst Appl Microbiol 33:328–336. doi: 10.1016/j.syapm.2010.07.004 CrossRefPubMedGoogle Scholar
- Felsenstein J (1993) PHYLIP (phylogeny interference package), 3.6a edn. University of Washington, SeattleGoogle Scholar
- Giammanco GM, Grimont PAD, Grimont F, Lefevre M, Giammanco G, Pignato S (2011) Phylogenetic analysis of the genera Proteus, Morganella and Providencia by comparison of rpoB gene sequences of type and clinical strains suggests the reclassification of Proteus myxofaciens in a new genus, Cosenzaea gen. nov., as Cosenzaea myxofaciens comb. nov. Int J Syst Evol Microbiol 61:1638–1644. doi: 10.1099/ijs.0.021964-0 CrossRefPubMedGoogle Scholar
- Mohan SB, Schmid M, Jetten M, Cole J (2004) Detection and widespread distribution of the nrfA gene encoding nitrite reduction to ammonia, a short circuit in the biological nitrogen cycle that competes with denitrification. FEMS Microbiol Ecol 49:433–443. doi: 10.1016/j.femsec.2004.04.012 CrossRefPubMedGoogle Scholar
- Richard C, Kiredjian M (1995) Laboratory methods for the identification of strictly aerobic gram-negative bacilli. Institut Pasteur, ParisGoogle Scholar
- Simon J (2011) Organization of respiratory electron transport chains in nitrate-reducing and nitrifying bacteria. In: Moir JWB (ed) Nitrogen cycling in bacteria: molecular analysis. Caister Academic Press, Norfolk, pp 39–58Google Scholar
- Stein LY (2011) Surveying N2O-producing pathways in bacteria. In: Klotz MG (ed) Methods in Enzymology, vol 486. Academic Press, Burlington, pp 131–152Google Scholar