Antonie van Leeuwenhoek

, Volume 108, Issue 5, pp 1205–1212 | Cite as

Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond

  • Feng-Qing Wang
  • Zhi-Hong Xie
  • Jin-Xin Zhao
  • Guan-Jun Chen
  • Zong-Jun Du
Original Paper

Abstract

A yellow-pigmented, Gram-stain negative and facultatively anaerobic bacterium, designated MF326T, was isolated from a sample of sediment collected from a sea cucumber culture pond in Rongcheng, China (122°14′34″E 36°54′36″N). Cells of strain MF326T were found to be catalase negative and oxidase positive. Optimal growth was found to occur at 30 °C and pH 7.0–7.5 in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MF326T is a member of the genus Mesonia and exhibits the high sequence similarity (94.3 %) with the type strain of Mesonia ostreae, followed by Mesonia algae (93.9 %). The dominant fatty acids of strain MF326T were identified as iso-C15:0, an unidentified fatty acid with an equivalent chain-length of 13.565 and anteiso-C15:0. The major polar lipids were found to be two unidentified lipids and phosphatidylethanolamine. The major respiratory quinone was found to be MK-6 and the genomic DNA G+C content was determined to be 40.7 mol%. On the basis of the phylogenetic analysis and differential phenotypic characteristics, it is concluded that strain MF326T (=KCTC 42255T =MCCC 1H00125T) should be assigned to the genus Mesonia as the type strain of a novel species, for which the name Mesonia sediminis sp. nov. is proposed.

Keywords

Mesonia sediminis sp. nov. 16S rRNA gene Polyphasic taxonomy Sediment 

Supplementary material

10482_2015_575_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 12 kb)
10482_2015_575_MOESM2_ESM.docx (112 kb)
Supplementary material 2 (DOCX 112 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Feng-Qing Wang
    • 1
  • Zhi-Hong Xie
    • 2
  • Jin-Xin Zhao
    • 1
  • Guan-Jun Chen
    • 1
    • 3
  • Zong-Jun Du
    • 1
    • 3
  1. 1.College of Marine ScienceShandong University at WeihaiWeihaiChina
  2. 2.Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantaiChina
  3. 3.State key Laboratory of Microbial TechnologyShandong UniversityJinanChina

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