Antonie van Leeuwenhoek

, Volume 108, Issue 4, pp 1009–1014 | Cite as

Hyunsoonleella udoensis sp. nov., isolated from a gravel sample from a beach of Udo island, Korea

  • Hyangmi Kim
  • Jae-Bong Lee
  • Hyun-Woo Oh
  • Hyung-Kee Cha
  • Jae-Hyung Yang
  • Kyung Sook Bae
  • Doo-Sang Park
Original Paper


A Gram-stain negative, non-motile, rod-shaped and aerobic bacterial strain, designated JG48T, was isolated from a gravel sample taken from a beach adjacent to Udo island, South Korea. Strain JG48T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The 16S rRNA gene sequence of strain JG48T exhibited sequence similarities of 96.67 % to Hyunsoonleella jejuensis CNU004T. The major fatty acids present in the strain JG48T were identified as iso-C15:0, iso-C15:1 G, iso-C17:0 3–OH and iso-C15:0 3–OH. The predominant isoprenoid quinone was identified as MK-6. The polar lipids profile of strain JG48T was found to consist of phosphatidylethanolamine, three unidentified amino lipids and four unidentified lipids. The DNA G+C content of strain JG48T was determined to be 34 mol%. Based on the morphological and physiological properties, and the results of phylogenetic analyses, the strain is considered to represent a novel species of the genus Hyunsoonleella, for which the name Hyunsoonleella udoensis sp. nov. is proposed. The type strain is JG48T (=KCTC 42341T=JCM 30600T).


Hyunsoonleella udoensis Flavobacteriaceae Seawater bacteria 



This work was supported by a grant from the National Fisheries Research and Development Institute (R2015028), and a Grant from the KRIBB Research Initiative Program (KGM4111551).

Supplementary material

10482_2015_553_MOESM1_ESM.pdf (539 kb)
Supplementary material (PDF 538 kb)


  1. Bernardet JF, Nakagawa Y (2006) An introduction to the family Flavobacteriaceae. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes: a handbook on the biology of bacteria, vol 7, 3rd edn. Springer, New York, pp 455–480CrossRefGoogle Scholar
  2. Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070PubMedGoogle Scholar
  3. Fautz E, Reichenbach H (1980) A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91CrossRefGoogle Scholar
  4. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  5. Felsenstein J (1993) PHYLIP (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, SeattleGoogle Scholar
  6. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  7. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefPubMedGoogle Scholar
  8. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  9. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Patel PV, Alshamaony L (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117CrossRefGoogle Scholar
  10. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  11. Moore DD, Dowhan D (1995) Preparation and analysis of DNA. In: Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, New York, pp 2–11Google Scholar
  12. Park SC, Choe HN, Hwang YM, Baik KS, Kim SN, Lee YS, Jung JS, Seong CN (2013) Marinivirga aestuarii gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from marine environments, and emended descriptions of the genera Hyunsoonleella, Jejuia and Pontirhabdus and the species Hyunsoonleella jejuensis, Jejuia pallidilutea and Pontirhabdus pectinivorans. Int J Syst Evol Microbiol 63:1524–1531CrossRefPubMedGoogle Scholar
  13. Reichenbach H (1989) The order Cytophagales Leadbetter 1974, 99AL. In: Staley JT, Bryant MP, Pfennig N, Holt JC (eds) Bergey’s manual of systematic bacteriology, vol 3. Williams and Wilkins, Baltimore, pp 2011–2073Google Scholar
  14. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  15. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  16. Sasser M (2001) Identification of bacteria by gas chromatography of cellular fatty acids. Technical note 101. MIDI Inc., NewarkGoogle Scholar
  17. Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654Google Scholar
  18. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729PubMedCentralCrossRefPubMedGoogle Scholar
  19. Tittsler RP, Sandholzer LA (1936) The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580PubMedCentralPubMedGoogle Scholar
  20. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703PubMedCentralPubMedGoogle Scholar
  21. Yoon BJ, Lee DH, Kang BJ, Kahng HY, Oh YS, Sohn JH, Choi ES, Oh DC (2010) Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 60:382–386CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Hyangmi Kim
    • 1
  • Jae-Bong Lee
    • 4
  • Hyun-Woo Oh
    • 2
  • Hyung-Kee Cha
    • 4
  • Jae-Hyung Yang
    • 3
  • Kyung Sook Bae
    • 1
  • Doo-Sang Park
    • 1
    • 5
  1. 1.Microbiological Resources CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonRepublic of Korea
  2. 2.Industrial Bio-Materials Resources CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonRepublic of Korea
  3. 3.Dokdo Fisheries Research CenterNational Fisheries Research and Development InstitutePohangRepublic of Korea
  4. 4.Fisheries Resources Management DivisionNational Fisheries Research and Development InstituteBusanRepublic of Korea
  5. 5.Department of Green Chemistry and Environmental BiotechnologyThe University of Science and Technology (UST)DaejeonRepublic of Korea

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