Antonie van Leeuwenhoek

, Volume 108, Issue 3, pp 721–729 | Cite as

Nitrospirillum irinus sp. nov., a diazotrophic bacterium isolated from the rhizosphere soil of Iris and emended description of the genus Nitrospirillum

  • Eu Jin Chung
  • Tae Soon Park
  • Kyung Hyun Kim
  • Che Ok Jeon
  • Hae-In Lee
  • Woo-Suk Chang
  • Zubair Aslam
  • Young Ryun Chung
Original Paper

Abstract

A polyphasic approach was used to characterize a novel nitrogen-fixing bacterial strain, designated YC6995T, isolated from the rhizosphere soil of Iris ensata var. spontanea (Makino) Nakai inhabiting a wetland located at an altitude of 960 m on Jiri Mountain, Korea. Strain YC6995T cells were Gram-negative, and rod-shaped, with motility provided by a single polar flagellum. Optimal growth conditions were 30 °C and pH 7.0. The major fatty acids of strain YC6995T were C18:1ω7c, C18:1 2-OH and C16:0 3-OH. The major respiratory quinone was ubiquinone-10 (Q-10). The polar lipids were phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, phosphatidylglycerol and unidentified glycolipids. The genomic DNA G+C content was 64.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed strain YC6995T to form a phyletic lineage with Nitrospirillum amazonense DSM 2787T with a high sequence similarity (97.2 %), but it displayed low sequence similarity with other remotely related genera, including Azospirillum (<93 %), Rhodocista (93.1–93.4 %), and Skermanella (91.2–93.3 %) in the family Alphaproteobacteria. Based on the phenotypic, chemotaxonomic, and phylogenetic evidences, strain YC6995T represents a novel species within the genus Nitrospirillum, for which the name Nitrospirillum irinus sp. nov. is proposed. The type strain is YC6995T (= KACC 13777T = DSM 22198T). An emended description of the genus Nitrospirillum is also proposed.

Keywords

Proteobacteria Nitrogen-fixing bacteria Nitrospirillum irinus sp. nov 

Notes

Acknowledgments

This work was carried out with the partial support of “Cooperative Research Program for Agriculture Science & Technology Development (PJ 01104901)” Rural Development Administration, Republic of Korea. E. J. Chung was supported by a scholarship from the BK21 Plus Program, the Ministry of Education, Republic of Korea.

Supplementary material

10482_2015_528_MOESM1_ESM.docx (2.5 mb)
Supplementary figures of the transmission electron micrograph and polar lipid analysis of strain YC6995T are available online. (DOCX 2565 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Eu Jin Chung
    • 1
  • Tae Soon Park
    • 1
  • Kyung Hyun Kim
    • 2
  • Che Ok Jeon
    • 2
  • Hae-In Lee
    • 3
  • Woo-Suk Chang
    • 3
    • 4
  • Zubair Aslam
    • 5
  • Young Ryun Chung
    • 1
  1. 1.Division of Applied Life Science (BK 21 Plus), Plant Molecular Biology & Biotechnology Research CenterGyeongsang National UniversityJinjuRepublic of Korea
  2. 2.Department of Life ScienceChung-Ang UniversitySeoulRepublic of Korea
  3. 3.Department of BiologyUniversity of Texas-ArlingtonArlingtonUSA
  4. 4.Division of Biotechnology, College of Environmental and Bioresource SciencesChonbuk National UniversityIksanRepublic of Korea
  5. 5.Department of AgronomyUniversity of AgricultureFaisalabadPakistan

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