Antonie van Leeuwenhoek

, Volume 108, Issue 3, pp 695–701 | Cite as

Hydrogenophaga luteola sp. nov. isolated from reed pond water

  • Juan Du
  • Jung-Eun Yang
  • Hina Singh
  • Shahina Akter
  • KyungHwa Won
  • Chang Shik Yin
  • Feng-Xie Jin
  • Tae-Hoo Yi
Original Paper

Abstract

A yellowish colored, Gram-staining negative, strictly aerobic, motile, rod-shaped bacterium, designated THG-SQE7T, was isolated from reed pond water in Shangqiu, PR China. Comparative 16S rRNA gene sequence analysis indicated that strain THG-SQE7T is most closely related to Hydrogenophaga pseudoflava ATCC 33668T (98.4 %), followed by Hydrogenophaga bisanensis K102T (97.6 %) and Hydrogenophaga flava CCUG 1658T (97.6 %). DNA–DNA hybridization showed 53.5, 36.0 and 22.5 % DNA re-association with H. pseudoflava KCTC 2348T, H. bisanensis KCTC 12980T and H. flava KCTC 1648T, respectively. Chemotaxonomic data revealed that strain THG-SQE7T possesses ubiquinone-8 as the only isoprenoid quinone, summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0 and C18:1ω7c as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was determined to be 63.7 mol%. These data corroborated the affiliation of strain THG-SQE7T to the genus Hydrogenophaga. Thus, the isolate represents a novel species, for which the name Hydrogenophagaluteola sp. nov. is proposed, with THG-SQE7T as the type strain (=KCTC 42501T = CCTCC AB 2014314T = JCM 30433T).

Keywords

Hydrogenophaga luteola Gram-staining negative Ubiquinone-8 16S rRNA 

Notes

Acknowledgments

This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).

Supplementary material

10482_2015_525_MOESM1_ESM.pdf (14 kb)
Supplementary material 1 (PDF 13 kb) Supplementary Fig. S1. Maximum likelihood tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships between strain THG–SQE7T and related members of the genus Hydrogenophaga. Numbers at nodes represent percentages of bootstrap support based on a maximum–likelihood analysis of 1,000 resampled datasets. Symbol * indicated the non-valid species of genus Hydrogenophaga
10482_2015_525_MOESM2_ESM.pdf (189 kb)
Supplementary material 2 (PDF 188 kb) Supplementary Fig. S2. Transmission electron micrograph of cells of strain THG–SQE7T. The detection was performed after negative staining with uranyl acetate. Bar, 0.5µm
10482_2015_525_MOESM3_ESM.pdf (273 kb)
Supplementary material 3 (PDF 272 kb) Supplementary Fig. S3. Two-dimensional TLC of the polar lipids of THG–SQE7T (a) and H. pseudoflava KCTC 2348T (b). a1 and b1: total lipids revealed by spraying with 5% molybdatophosphoric acid for strain THG–SQE7T and H. pseudoflava KCTC 2348T, respectively; a2 and b2: aminolipid revealed by spraying with 0.2% ninhydrin of stain THG–SQE7T and H. pseudoflava KCTC 2348T, respectively; a3 and b3: phospholipid revealed by molybdenum blue of stain THG–SQE7T and H. pseudoflava KCTC 2348T, respectively. No glycolipid appeared. Abbreviations: phosphatidylethanolamine (PE), diphosphatidylglycerol (DPE), phosphatidylglycerol (PG), unidentified phospholipid (PL) and unidentified lipids (L0-L5)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Juan Du
    • 1
  • Jung-Eun Yang
    • 1
  • Hina Singh
    • 1
  • Shahina Akter
    • 1
  • KyungHwa Won
    • 1
  • Chang Shik Yin
    • 2
  • Feng-Xie Jin
    • 3
  • Tae-Hoo Yi
    • 1
  1. 1.Department of Oriental Medicinal Biotechnology, College of Life ScienceKyung Hee UniversityYongin-siRepublic of Korea
  2. 2.Department of Acupuncture Merdian Science Research Center, College of Korean MedicineKyung Hee UniversitySeoulRepublic of Korea
  3. 3.College of Bio and Food TechnologyDalian Polytechnic UniversityDalianPeople’s Republic of China

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