Antonie van Leeuwenhoek

, Volume 108, Issue 3, pp 667–672 | Cite as

Umezawaea endophytica sp. nov., isolated from tobacco root samples

  • Xiao Chu
  • Bing-Bing Liu
  • Rui Gao
  • Zheng-Yu Zhang
  • Yan-Qing Duan
  • Salam Nimaichand
  • Wei Chen
  • Wen-Jun Li
Original Paper

Abstract

Two Gram-staining positive, aerobic and non-motile actinomycete strains, designated YIM 2047XT and YIM 2047D, were isolated from tobacco root samples collected from Shiling county, Yunnan province, South-West China. The isolates grew at 15–40 °C, pH 6.0–8.0 and 0–4.5 % NaCl (w/v). The whole-cell hydrolysates contained meso-diaminopimelic acid, galactose, mannose, ribose and rhamnose. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major cellar fatty acids identified were iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0, C17:1 ω8c and C17:0. MK-9 (H4) was the predominant menaquinone. The genomic DNA G+C contents were 73.4 and 74.2 mol%, respectively. These chemotaxonomic data, together with their morphological properties, were consistent with the assignment of the two strains to the genus Umezawaea. They showed highest similarities to Umezawaea tangerina JCM 10302T on phylogenetic analysis of their 16S rRNA gene sequences and were found to form a coherent cluster. However, the DNA–DNA hybridization values of YIM 2047XT and YIM 2047D with U. tangerina JCM 10302T were 46.5 ± 3.1 and 57.5 ± 1.6 %, respectively; while the reassociation value between themselves was 80.4 ± 2.3 %. The results of physiological and biochemical tests allowed the two new isolates to be differentiated phenotypically from the recognized strain U. tangerina JCM 10302T. On the basis of results from this polyphasic study, the strains were characterized as a novel species of the genus Umezawaea, for which the name Umezawaea endophytica sp. nov. is proposed. The type strain is YIM 2047XT (=KCTC 39538T = CPCC 204132T).

Keywords

Umezawaea endophytica sp. nov. Polyphasic taxonomy Tobacco Yunnan province 

Notes

Acknowledgments

We are grateful to Prof. Dr. Takuji Kudo (JCM) for providing the reference type strain. This research was supported by projects of China tobacco Yunnan Industrial Co. Ltd. (Nos. 2012JC07, 2012FL02 and 2014YL01). W-J Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

Supplementary material

10482_2015_522_MOESM1_ESM.doc (2.3 mb)
Supplementary material 1 (DOC 2406 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Xiao Chu
    • 1
  • Bing-Bing Liu
    • 1
  • Rui Gao
    • 2
  • Zheng-Yu Zhang
    • 3
  • Yan-Qing Duan
    • 2
  • Salam Nimaichand
    • 4
  • Wei Chen
    • 2
  • Wen-Jun Li
    • 1
    • 4
  1. 1.Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China
  2. 2.China Tobacco Yunnan Industrial Co., LtdKunmingPeople’s Republic of China
  3. 3.Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Ministry of EducationYunnan Agricultural UniversityKunmingPeople’s Republic of China
  4. 4.State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and EvolutionSun Yat-Sen UniversityGuangzhouPeople’s Republic of China

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