Antonie van Leeuwenhoek

, Volume 108, Issue 3, pp 659–666 | Cite as

Paenibacillus populi sp. nov., a novel bacterium isolated from the rhizosphere of Populus alba

  • Tong-Yan Han
  • Xiao-Mei Tong
  • Yan-Wei Wang
  • Hui-Min Wang
  • Xiao-Rong Chen
  • De-Long Kong
  • Xiang Guo
  • Zhi-Yong Ruan
Original Paper

Abstract

A novel aerobic bacterium, designated strain LAM0705T, was isolated from the rhizosphere of Populus alba in the Peking University Third Hospital. Cells of strain LAM0705T were observed to be Gram-stain positive, motile, spore-forming and rod-shaped. The optimal temperature and pH for growth were found to be 30 °C and pH 7.5, respectively. Strain LAM0705T was found to be able to grow in the presence 0–5 % NaCl (w/v) (optimum 1.0 %). The major fatty acids of strain LAM0705T were identified as anteiso-C15:0, C16:0 and iso-C16:0. The dominant polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan of strain LAM0705T was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The G+C content of genomic DNA was found to be 48 mol% when determined by the Tm method. The 16S rRNA gene sequence similarity analysis indicated that strain LAM0705T is closely related to Paenibacillus agaridevorans DSM 1355T and Paenibacillus thailandensis KCTC 13043T with 97.8 and 96.1 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0705T and P. agaridevorans DSM 1355T was 47 ± 0.8 %. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0705T is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus populi sp. nov. is proposed. The type strain is LAM0705T (=ACCC 06427T = JCM 19843T).

Keywords

Paenibacillus populi sp. nov. Polyphasic taxonomy 16S rRNA gene Rhizosphere Populus alba 

Notes

Acknowledgments

This work was supported by National Nonprofit Institute Research Grant of CAAS (No. 2014-30), National Key Technology R&D Program of China (No. 2013BAD05B04F02 and 2011BAD11B05), Foundation of the Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China) (No. 2013002), Science Foundation of Modern Farming Group (No. MF20100518), and Science Foundation of Dajing Group.

Supplementary material

10482_2015_521_MOESM1_ESM.pdf (820 kb)
Supplementary material 1 (PDF 820 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Tong-Yan Han
    • 1
  • Xiao-Mei Tong
    • 1
  • Yan-Wei Wang
    • 2
  • Hui-Min Wang
    • 2
  • Xiao-Rong Chen
    • 2
  • De-Long Kong
    • 2
  • Xiang Guo
    • 2
  • Zhi-Yong Ruan
    • 2
  1. 1.Pediatric DepartmentPeking University Third HospitalBeijingChina
  2. 2.Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional PlanningCAASBeijingChina

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