Antonie van Leeuwenhoek

, Volume 108, Issue 3, pp 649–658 | Cite as

Marinobacter adhaerens HP15 harbors two CzcCBA efflux pumps involved in zinc detoxification

  • Antje Stahl
  • Daniel Pletzer
  • Amna Mehmood
  • Matthias S. UllrichEmail author
Original Paper


Several members of the ubiquitously found γ-proteobacterial genus Marinobacter were described or assumed to inhabit marine environments naturally enriched in heavy metals. However, direct studies that describe the ability of this genus to occupy such environments have not been conducted. To cope with heavy metal stress, bacteria possess specific efflux pumps as tools for detoxification, among which the CzcCBA type efflux system is one representative. Previous studies showed that this system plays an important role in resistance towards cadmium, zinc, and cobalt. Up to now, no study had focused on characterization of Czc pumps in Marinobacter sp. or other marine prokaryotes. Herein, we elucidated the function of two CzcCBA pumps encoded by Marinobacter adhaerens HP15’s genome during exposure to cadmium, zinc, and cobalt. Single and double knock-out mutants lacking the corresponding two czcCBA operons were generated and analyzed in terms of their resistance profiles. Both operons appeared to be important for zinc resistance but had no role in tolerance towards cadmium or cobalt. One of the mutations was genetically complemented thereby restoring the wild type phenotype. In accordance with the resistance pattern, expression of the genes coding for both CzcCBA pumps was induced by zinc but neither by cadmium nor cobalt.


CzcA Multidrug efflux pump Cupriavidus metallidurans CH34 Zinc Heavy metal resistance Czc operon 



This project was funded by the Helmholtz Graduate School for Polar and Marine Research (POLMAR) and Deutsche Forschungsgemeinschaft (UL 169/6-1). The authors would like to thank Dr. Helge Weingart for excellent technical suggestions on mutant generation and Desalegne Abebew Syit for help with cloning of knock-out plasmids.

Supplementary material

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Supplementary material 1 (DOCX 17 kb)
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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Antje Stahl
    • 1
  • Daniel Pletzer
    • 1
  • Amna Mehmood
    • 1
  • Matthias S. Ullrich
    • 1
    Email author
  1. 1.Department of Life Sciences and ChemistryJacobs University BremenBremenGermany

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