Paralcaligenes ginsengisoli sp. nov., isolated from ginseng cultivated soil
A novel bacterial strain DCY104T isolated from soil of a ginseng field in Yeoncheon County, Republic of Korea is described in this study. Cells were short rod-shaped, motile by mean of one polar flagellum, strictly aerobic, Gramreaction negative, oxidase and catalase-positive. 16S rRNA gene sequence analysis showed that strain DCY104T shared highest similarity 98.2 % to Paralcaligenes ureilyticus GR24-5T, and from 97.7 to 97.1 % with other type strains belong to the genera Candidimonas, Pusillimonas and Parapusillimonas Otherwise, phylogenetic trees analyses indicated that strain DCY104T belongs to a single group with P. ureilyticus GR24-5T that was distinct to other genera. The major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The major cellular fatty acids consisted of C16:0, cyclo-C17:0, and summed feature 8 (comprising C18:1ω7c and/or C18:1ω6c). The predominant polyamine was putrescine. The ubiquinone was Q-8. The genomic DNA G+C content was 55.9 mol%. These data in combination with the presence of one polar flagellum and positive activity of urease confirmed the placement of strain DCY104T in the genus Paralcaligenes. DNA–DNA relatedness between strain DCY104T and P. ureilyticus KACC 13888T was 40 %. The differences in the profiles of polar lipids, fatty acids and phenotypic characteristics in combination with DNA–DNA relatedness delineated strain DCY104T and P. ureilyticus KACC 13888T. In summary, taxonomic analyses in this study demonstrated that strain DCY104T represents a novel species within the genus Paralcaligenes, for which we propose the name Paralcaligenes ginsengisoli. The type strain is DCY104T (= KCTC 42406T = JCM 30746T).
KeywordsParalcaligenes ginsengisoli Ginseng soil One polar flagellum
This research was supported by Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (KIPET No: 313038-03-1-HD030).
- Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
- Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden TL (2008) NCBI BLAST: a better web interface. Nucleic Acids Res 1:36 (Web Server issue):W5-9. doi: 10.1093/nar/gkn201
- Lane JD (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
- Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital capacity of source microbial species. Microbiologiya 17:362–370Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, NewarkGoogle Scholar