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Antonie van Leeuwenhoek

, Volume 108, Issue 3, pp 571–577 | Cite as

Pseudonocardia nematodicida sp. nov., isolated from mangrove sediment in Hainan, China

  • Min Liu
  • Shan-shan Xing
  • Wei-dao Yuan
  • Hua Wei
  • Qian-guang Sun
  • Xiang-zhi Lin
  • Hui-qin HuangEmail author
  • Shi-xiang BaoEmail author
Original Paper

Abstract

Two aerobic, Gram-stain positive actinobacterial strains with nematicidal activity, designated HA11164T and HA12591, were isolated from mangrove sediments in Hainan, China. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strains HA11164T and HA12591 belong to the genus Pseudonocardia and are closely related to Pseudonocardia carboxydivorans (with the similarities of 98.30 and 98.24 %, respectively), Pseudonocardia alni (98.23 and 98.16 %, respectively) and Pseudonocardia antimicrobica (98.10 and 98.03 %, respectively). The major polar lipids of the strain HA11164T, as a representative strain of the two strains, were found to consist of phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, five unidentified glycolipids and four unidentified polar lipids. The predominant menaquinone of strain HA11164T was identified as MK-8 (H4), and the major fatty acids were identified as iso-C16:0, C17:1 ω10, C16:0 and C16:1 ω9. The G+C content of strain HA11164T was determined to be 74.9 mol%. The DNA–DNA relatedness values between strains HA11164T and P. alni, Pseudonocardia tropica, Pseudonocardia antarctica, P. carboxydivorans and Pseudonocardia parietis were 58.3, 56.2, 50.0, 57.1 and 46.0 %, respectively. Based on the results of this polyphasic study, strains HA11164T and HA12591 are considered to represent a novel species of the genus Pseudonocardia, for which the name Pseudonocardia nematodicida sp. nov. is proposed. The type strain is HA11164T (=CGMCC 4.7118T = DSM 45940T).

Keywords

Pseudonocardia nematodicida sp. nov. 16S rRNA gene Polyphasic taxonomy 

Notes

Acknowledgments

This research was supported by National Natural Science Foundation of China (31400010, 31170062), National Basic Research Program of China (973 Program) (2013CB127500), National Project of Marine economy innovation development area demonstration (12PYY001SF08–ZGRKY–1), Hainan major technology project (ZDZX2013023-1), Special Fund for Agro-scientific Research in the Public Interest (201403075) and National Non-profit Institute Research Grant of CATAS-ITBB from Chinese Government (1630052012002, 1630052013004).

Supplementary material

10482_2015_512_MOESM1_ESM.doc (512 kb)
Supplementary material 1 (DOC 511 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Min Liu
    • 1
  • Shan-shan Xing
    • 1
  • Wei-dao Yuan
    • 1
  • Hua Wei
    • 1
  • Qian-guang Sun
    • 1
  • Xiang-zhi Lin
    • 2
  • Hui-qin Huang
    • 1
    Email author
  • Shi-xiang Bao
    • 1
    Email author
  1. 1.Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Institute of Tropical Biosciences and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouPeople’s Republic of China
  2. 2.Key Laboratory of Marine Biogenetic Resources, Third Institute of OceanographyState Oceanic AdministrationXiamenPeople’s Republic of China

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