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Antonie van Leeuwenhoek

, Volume 108, Issue 3, pp 553–561 | Cite as

Lysobacter agri sp. nov., a bacterium isolated from soil

  • Hina Singh
  • KyungHwa Won
  • Juan Du
  • Jung-Eun Yang
  • Shahina Akter
  • Ki-Young Kim
  • Tae-Hoo YiEmail author
Original Paper

Abstract

A bacterial strain, designated as THG-SKA3T, was isolated from field soil of Kyung Hee University, South Korea. Cells of the isolate were observed to be Gram-negative, aerobic, rod-shaped and motile by gliding. The strain was found to grow optimally at 28 °C, at pH 7 and in absence of NaCl. Based on 16S rRNA gene sequence comparisons, strain THG-SKA3T shared highest sequence similarity with Lysobacter niastensis KACC 11588T followed by Lysobacter panacisoli KACC 17502T, Lysobacter enzymogenes LMG 8762T and Lysobacter oryzae KCTC 22249T. The G+C content of THG-SKA3T was determined to be 68.9 mol%. The DNA–DNA relatedness values between strain THG-SKA3T and its closest phylogenetic neighbors were below 25.0 %.The major polar lipids of strain THG-SKA3T were determined to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was identified as ubiquinone 8 (Q-8). The major cellular fatty acids were identified as branched chain iso-C15:0, iso-C16:0 and unsaturated iso-C17:1 ω9c. On the basis of polyphasic data presented, it is evident that strain THG-SKA3T represents a novel species of the genus Lysobacter, for which the name Lysobacter agri sp. nov. (type strain THG-SKA3T = KACC 18283T = CSCTCC AB 2015126T) is proposed.

Keywords

Lysobacter agri Polyphasic taxonomy Gram-negative Ubiquinone Q-8 

Notes

Acknowledgments

This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).

Supplementary material

10482_2015_510_MOESM1_ESM.tif (109 kb)
Supplementary Fig. S1 The maximum-likelihood tree based on 16S rRNA gene sequence analysis showing the phylogenetic relationships between strain THG-SKA3T and members of the genus Lysobacter. Bootstrap values less than 50 % were not indicated. Dyella terrae JS14-6T was used as an out group. Scale bar, 0.05 substitutions per nucleotide position. Supplementary material 1 (TIFF 109 kb)
10482_2015_510_MOESM2_ESM.tif (1.7 mb)
Supplementary Fig. S2. Transmission electron micrograph of Lysobacter agri THG-SKA3T. Bar indicated 0.5 μm. Supplementary material 2 (TIFF 1768 kb)
10482_2015_510_MOESM3_ESM.tif (1.6 mb)
Supplementary Fig. S3. Two-dimensional TLC of the total polar lipids of Lysobacter agri THG-SKA3T (a) and Lysobacter niatensis KACC 11588T (b), stained for total polar lipids with 5 % ethanolic molybdatophosphoric acid. Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; APL1-2, unidentified aminophospholipid; PL, unidentified phospholipid. Supplementary material 3 (TIFF 1609 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Hina Singh
    • 1
  • KyungHwa Won
    • 1
  • Juan Du
    • 1
  • Jung-Eun Yang
    • 1
  • Shahina Akter
    • 1
  • Ki-Young Kim
    • 2
  • Tae-Hoo Yi
    • 1
    Email author
  1. 1.Department of Oriental Medicine Biotechnology, College of Life ScienceKyung Hee University Global CampusYongin-siRepublic of Korea
  2. 2.Department of Genetic Engineering, College of Life ScienceKyung Hee University Global CampusYongin-siRepublic of Korea

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