Halosimplex litoreum sp. nov., isolated from a marine solar saltern
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A halophilic archaeal strain, YGH94T, was isolated from the Yinggehai marine solar saltern near the Shanya city of Hainan Province, China. Cells of the strain were observed to be short rods, stain Gram-negative and to form red-pigmented colonies on solid media. Strain YGH94T was found to grow at 25–50 °C (optimum 40 °C), at 0.9–4.8 M NaCl (optimum 3.1 M), at 0–1.0 M MgCl2 (optimum 0.05 M) and at pH 5.0–9.0 (optimum pH 7.5). The cells were found to lyse in distilled water and the minimal NaCl concentration to prevent cell-lysis was determined to be 5 % (w/v). The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and four major glycolipids (disulfated mannosyl glucosyl diether, sulfated mannosyl glucosyl diether and two unidentified glycolipids chromatographically identical to glycolipids in Halosimplex carlsbadense JCM 11222T). Strain YGH94T was found to possess two heterogeneous 16S rRNA genes (rrnA and rrnB) and both are related to those of Hsx. carlsbadense JCM 11222T (92.7–98.6 % similarities), Halosimplex pelagicum R2T (94.6–99.2 % similarities) and Halosimplex rubrum R27T (92.9–98.8 % similarities). The rpoB′ gene similarity between strain YGH94T and Hsx. carlsbadense JCM 11222T, Hsx. pelagicum R2T and Hsx. rubrum R27T are 95.4, 94.9 and 95.1 %, respectively. The DNA G+C content of strain YGH94T was determined to be 64.0 mol%. Strain YGH94T showed low DNA–DNA relatedness (35–39 %) with the current three members of the genus Halosimplex. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain YGH94T (=CGMCC 1.12235T = JCM 18647T) represents a new species of the genus Halosimplex, for which the name Halosimplexlitoreum sp. nov. is proposed.
KeywordsHalosimplexlitoreum sp. nov. Halophilic archaeon Marine solar saltern Polyphasic taxonomy
This work was supported by the National Natural Science Foundation of China (No. 31370054), the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021), the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
- Koh H-W, Song HS, Song U, Yim KJ, Roh SW, Park S-J (2015) Halolamina sediminis sp. nov., an extremely halophilic archaeon isolated from solar salt. Int J Syst Evol Microbiol (in press)Google Scholar
- Kondo Y, Minegishi H, Echigo A, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Takahashi-Ando N, Fukushima Y, Yoshida Y, Usami R (2015). Halorubrum gandharaense sp. nov., a novel alkaliphilic haloarchaeon from commercial rock salt. Int J Syst Evol Microbiol (in press)Google Scholar
- Minegishi H, Echigo A, Kuwahara A, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Usami R (2015a) Halocalculus aciditolerans gen. nov., sp. nov., an acid-tolerant haloarchaeon isolated from commercial salt. Int J Syst Evol Microbiol (in press)Google Scholar
- Minegishi H, Echigo A, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Usami R (2015b) Halococcus agarilyticus sp. nov., an agar degrading haloarchaeon isolated from commercial salt. Int J Syst Evol Microbiol (in press)Google Scholar
- Owen RJ, Pitcher D (1985) Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 67–93Google Scholar
- Vreeland RH, Straight S, Krammes J, Dougherty K, Rosenzweig WD, Kamekura M (2002) Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles 6:445–452PubMedCrossRefGoogle Scholar
- Zhang W-J, Cui H-L (2015) Halomicroarcula salina sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol (in press)Google Scholar