Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China
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A novel aerobic bacterium, KC1T, was isolated from a salt mine in Kuche county, Xinjiang province, China. Cells were observed to be Gram-positive, rod-shaped, endospore-forming and motile with flagella. Strain KC1T was found to grow at 25–45 °C (optimum 37 °C), pH 6.5–9.0 (optimum 8.0) and NaCl 0–10 % (v/v) (optimum 4 %). The major fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. Menaquinone-7 (MK-7) was found to be the predominant isoprenoid quinone. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol and a glycolipid. The 16S rRNA gene sequence of strain KC1T showed low similarity (<96 %) to other validly named species. The phylogenetic trees showed that strain KC1T is closely related to Bacillus azotoformans DSM 1046T and Bacillus methanolicus DSM 16454T. Both these type strains showed 95.4 % 16S rRNA gene sequence similarity to strain KC1T. The DNA G+C content of strain KC1T was determined to be 39.0 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KC1T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus salitolerans sp. nov. is proposed. The type strain is KC1T (=JCM 19760T = CGMCC 1.12810T).
KeywordsBacillus salitolerans sp. nov. Polyphasic taxonomy 16S rRNA gene Salt mine
This work was supported by grants from the National Natural Science Foundation of China (No. 31170001).
- Claus D, Berkeley CW (1986) The genus Bacillus. In: Sneath PHA (ed) Bergey’s manual of systematic bacteriology, vol 2. Williams and Wilkins, Baltimore, pp 1105–1139Google Scholar
- Cohn F (1872) Untersuchungen über bacterien. Beitr Biol Pflanz 1:127–244 (in German) Google Scholar
- Dong XZ, Cai MY (2001) Determination of biochemical properties. In: Dong XZ, Cai MY (eds) Manual for the systematic identification of general bacteria. Science Press, Beijing (in Chinese), pp 370–398Google Scholar
- Nazina TN, Lebedeva EV, Poltaraus AB, Tourova TP, Grigoryan AA, Sokolova DSH, Lysenko AM, Osipov GA (2004) Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov. Int J Syst Evol Microbiol 54:2019–2024PubMedCrossRefGoogle Scholar
- Priest FG (1993) Systematics and ecology of Bacillus. In: Sonenshein AL, Hoch JA, Losick R (eds) Bacillus subtilis and other gram-positive bacteria. American Society for Microbiology, Washington, DC, pp 3–33Google Scholar
- Shivaji S, Chaturvedi P, Suresh K, Reddy GSN, Dutt CBS, Wainwright M, Narlikar JV, Bhargava PM (2006) Bacillus aerius sp. nov., Bacillus aerophilus sp. nov., Bacillus stratosphericus sp. nov. and Bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. Int J Syst Evol Microbiol 56:1465–1473PubMedCrossRefGoogle Scholar
- Xu XW, Wu YH, Zhou Z, Wang CS, Zhou YG, Zhang HB, Wang Y, Wu M (2007) Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov., and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57:1619–1624PubMedCrossRefGoogle Scholar
- Zhu XF (2011) Modern experimental technique of microbiology. Zhejiang University Press (English translation), HangzhouGoogle Scholar