Antonie van Leeuwenhoek

, Volume 108, Issue 2, pp 443–451 | Cite as

Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China

  • Wei-Yan Zhang
  • Jing Hu
  • Xin-Qi Zhang
  • Xu-Fen Zhu
  • Min Wu
Original Paper

Abstract

A novel aerobic bacterium, KC1T, was isolated from a salt mine in Kuche county, Xinjiang province, China. Cells were observed to be Gram-positive, rod-shaped, endospore-forming and motile with flagella. Strain KC1T was found to grow at 25–45 °C (optimum 37 °C), pH 6.5–9.0 (optimum 8.0) and NaCl 0–10 % (v/v) (optimum 4 %). The major fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. Menaquinone-7 (MK-7) was found to be the predominant isoprenoid quinone. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol and a glycolipid. The 16S rRNA gene sequence of strain KC1T showed low similarity (<96 %) to other validly named species. The phylogenetic trees showed that strain KC1T is closely related to Bacillus azotoformans DSM 1046T and Bacillus methanolicus DSM 16454T. Both these type strains showed 95.4 % 16S rRNA gene sequence similarity to strain KC1T. The DNA G+C content of strain KC1T was determined to be 39.0 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KC1T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus salitolerans sp. nov. is proposed. The type strain is KC1T (=JCM 19760T = CGMCC 1.12810T).

Keywords

Bacillus salitolerans sp. nov. Polyphasic taxonomy 16S rRNA gene Salt mine 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 31170001).

Supplementary material

10482_2015_497_MOESM1_ESM.doc (1.3 mb)
Supplementary material 1 (DOC 1284 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Wei-Yan Zhang
    • 1
  • Jing Hu
    • 1
  • Xin-Qi Zhang
    • 2
  • Xu-Fen Zhu
    • 1
  • Min Wu
    • 1
  1. 1.College of Life SciencesZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.College of Forestry and BiotechnologyZhejiang Agricultural and Forestry UniversityLin’anPeople’s Republic of China

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