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Antonie van Leeuwenhoek

, Volume 108, Issue 2, pp 355–364 | Cite as

Vibrio mexicanus sp. nov., isolated from a cultured oyster Crassostrea corteziensis

  • Adrián González-Castillo
  • Julissa Enciso-Ibarrra
  • M. Carmen Bolán-Mejia
  • Sabela Balboa
  • Aide Lasa
  • Jesús L. Romalde
  • Hector Cabanillas-Beltrán
  • Bruno Gomez-GilEmail author
Original Paper

Abstract

A bacterial strain was taxonomically characterised by means of a genomic approach comprising 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), the DNA G+C content, whole genome analyses (ANI and GGDC) and phenotypic characterisation. The strain CAIM 1540T was isolated from a cultured oyster Crassostrea corteziensis in La Cruz, Sinaloa state, México. The isolate was found to be catalase and oxidase positive, cells were observed to be motile, O/129-sensitive and facultatively anaerobic. The almost-complete 16S rRNA gene sequence placed this strain within the genus Vibrio; the closest related species were found to be Vibrio aestivus, Vibrio marisflavi, Vibrio maritimus and Vibrio variabilis with similarity values of 99.02, 97.05, 96.70, and 96.59 % respectively. MLSA of four housekeeping genes (ftsZ, gapA, recA, and topA) was performed with the closely related species. A draft genome sequence of strain CAIM 1540T was obtained. The DNA G+C content of this strain was determined to be 43.7 mol%.The ANI values with V. aestivus were 89.6 % (ANIb), 90.6 % (ANIm) and a GGDC value of 39.5 ± 2.5 % was obtained; with V. marisflavi the genomic similarities were 71.5 % (ANIb), 85.5 % (ANIm) and 20.2 ± 2.3 % (GGDC); with V. maritimus 72.6 % (ANIb), 85.7 % (ANIm) and 22.0 ± 2.0 % (GGDC); and with V. variabilis 72.6 % (ANIb), 85.8 % (ANIm) and 21.6 ± 1.6 % (GGDC). These ANI and GGDC values are below the threshold for the delimitation of prokaryotic species, i.e. 95–96 and 70 %, respectively. Phenotypic characters also showed differences with the closest related species analysed. The results presented here support the description of a novel species, for which the name Vibrio mexicanus sp. nov. is proposed, with strain CAIM 1540T (= CECT 8828T, = DSM 100338T) as the type strain. In addition, we found that the recently described species Vibrio thalassae and Vibrio madracius might be a single species because the values of ANIb 95.8 %, ANIm 96.6 % and GGDC 70.2 ± 2.9 % are above the accepted species thresholds.

Keywords

Vibrio mexicanus Vibrio naceae Vibrio aestivus Vibrio marisflavi 

Abbreviations

ANI

Average nucleotide identity

GGDC

Genome-to-genome distance calculator

MLSA

Multilocus sequence analysis

Notes

Acknowledgments

This work was partially funded by CONACYT project CB-2009-01 132328 and Grant AGL–2010–18438 from the Ministerio de Ciencia e Innovación (Spain).

Supplementary material

10482_2015_488_MOESM1_ESM.doc (978 kb)
Supplementary material 1 (DOC 978 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Adrián González-Castillo
    • 1
  • Julissa Enciso-Ibarrra
    • 1
  • M. Carmen Bolán-Mejia
    • 1
  • Sabela Balboa
    • 2
  • Aide Lasa
    • 2
  • Jesús L. Romalde
    • 2
  • Hector Cabanillas-Beltrán
    • 3
  • Bruno Gomez-Gil
    • 1
    Email author
  1. 1.CIAD, A.C., Mazatlán Unit for AquacultureMazatlánMexico
  2. 2.Departamento de Microbiología y Parasitología, CIBUSUniversidad de Santiago de CompostelaSantiago de CompostelaSpain
  3. 3.Instituto Tecnológico de TepicTepicMexico

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