Antonie van Leeuwenhoek

, Volume 108, Issue 2, pp 301–310 | Cite as

Mesorhizobium soli sp. nov., a novel species isolated from the rhizosphere of Robinia pseudoacacia L. in South Korea by using a modified culture method

  • Tuan Manh Nguyen
  • Van Hong Thi Pham
  • Jaisoo Kim
Original Paper

Abstract

Strain NHI-8T was isolated from a forest soil sample, collected in South Korea, by using a modified culture method. Comparative analysis of its nearly full-length 16S rRNA gene sequence showed that strain NHI-8T belongs to the genus Mesorhizobium and to be closely related to Mesorhizobium chacoense PR5T (97.32 %). The levels of DNA–DNA relatedness between strain NHI-8T and reference type strains of the genus Mesorhizobium were 32.28–53.65 %. SDS-PAGE of total soluble proteins and the sequences of the housekeeping genes recA, glnII, and atpD were also used to support the clade grouping in rhizobia. The new strain contained summed feature 8 (57.0 %), cyclo-C19:0ω8c (17.3 %), and C18:0 (11.0 %) as the major fatty acids, as in genus Mesorhizobium. The strain contained cardiolipin, phosphatidylglycerol, ornithine-containing lipid, phosphatidylethanolamine, phosphatidyl-N-dimethylethanolamine, and phosphatidylcholine. Morphological and physiological analyses were performed to compare the characteristics of our strain with those of the reference type strains. Based on the results, strain NHI-8T was determined to represent a novel member of the genus Mesorhizobium, and the name Mesorhizobium soli is proposed. The type strain is NHI-8T (=KEMB 9005-153T = KACC 17916T = JCM 19897T).

Keywords

Mesorhizobium sp. nov. Housekeeping gene Taxonomy Modified culture method Rhizosphere Robinia pseudoacacia

Notes

Acknowledgments

This study was supported by the Bio-industry Technology Development Program (312027-3), Minstry of Agriculture, Food and Rural Affairs, and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology (2011-0010144).

Supplementary material

10482_2015_481_MOESM1_ESM.docx (980 kb)
Supplementary material 1 (DOCX 979 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Tuan Manh Nguyen
    • 1
  • Van Hong Thi Pham
    • 1
  • Jaisoo Kim
    • 1
  1. 1.Department of Life Science, College of Natural SciencesKyonggi UniversitySuwonRepublic of Korea

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