Antonie van Leeuwenhoek

, Volume 108, Issue 1, pp 133–139 | Cite as

Sphaerisporangium corydalis sp. nov., isolated from the root of Corydalis yanhusuo L.

  • Xin Wang
  • Chongxi Liu
  • Jiaxu Cheng
  • Yuejing Zhang
  • Zhaoxu Ma
  • Lianjie Li
  • Xiangjing Wang
  • Wensheng Xiang
Original Paper

Abstract

Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-YHS12 and NEAU-YHS15T, were isolated from the root of Corydalis yanhusuo L. collected from Wuchang, Heilongjiang Province, northeast China. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains are closely related to one another (99.8 % similarity), and had the closest relationship with Sphaerisporangium cinnabarinum JCM 3291T (98.7, 98.6 %), Sphaerisporangium flaviroseum YIM 48771T (98.6, 98.6 %), Sphaerisporangium melleum JCM 13064T (98.5, 98.4 %) and Sphaerisporangiumdianthi NEAU-CY18T (98.4, 98.4 %). DNA–DNA hybridization value between strains NEAU-YHS12 and NEAU-YHS15T was 82 ± 1.4 %, and the values between the two strains and the closely related type strains were well below 70 %. The two strains also shared a number of phenotypic characteristics that were distinct from the closely related species. Both strains were observed to contain MK-9(H6), MK-9(H4) and MK-9(H2) as the detected menaquinones. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The phospholipid profiles were found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphoglycolipid, and an unidentified phospholipid. The major fatty acids were identified as iso-C16:0, C17:1 ω7c, C18:0 and iso-C15:0. On the basis of the genetic and phenotypic properties, it is proposed that strains NEAU-YHS15T and NEAU-YHS12 be classified as representatives of a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium corydalis sp. nov is proposed. The type strain is NEAU-YHS15T (CGMCC 4.7148T = DSM46732T).

Keywords

Sphaerisporangium corydalis sp. nov Polyphasic taxonomy 16S rRNA gene 

Notes

Acknowledgments

This work was supported in part by Grants from the National Outstanding Youth Foundation (No. 31225024), the National Natural Science Foundation of China (No. 31471832, 31171913 and 31372006), the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent Talents in University (NCET-11-0953), the Outstanding Youth Foundation of Heilongjiang Province (JC201201), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the China Postdoctoral Science Foundation (2014M561319) and the Heilongjiang Postdoctoral Fund (LBH-Z14027).

Supplementary material

10482_2015_471_MOESM1_ESM.doc (704 kb)
Supplementary material 1 (DOC 704 kb)

References

  1. Ara I, Kudo T (2007) Sphaerosporangiumgen. nov., a new member of the family Streptosporangiaceae, with descriptions of three new species as Sphaerosporangium melleum sp. nov., Sphaerosporangium rubeum sp. nov. and Sphaerosporangium cinnabarinum sp. nov., and transfer of Streptosporangium viridialbum Nonomura and Ohara 1960 to Sphaerosporangium viridialbumcomb. nov. Actinomycetologica 21:11–21CrossRefGoogle Scholar
  2. Cao YR, Jiang Y, Xu LH, Jiang CL (2009) Sphaerisporangium flaviroseum sp. nov. and Sphaerisporangium album sp. nov., isolated from forest soil in China. Int J Syst Evol Microbiol 59:1679–1684PubMedCrossRefGoogle Scholar
  3. Collins MD (1985) Isoprenoid quinone analyses in bacterial classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 267–284Google Scholar
  4. De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142PubMedCrossRefGoogle Scholar
  5. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376PubMedCrossRefGoogle Scholar
  6. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  7. Gao RX, Liu CX, Zhao JW, Jia FY, Yu C, Yang LY, Wang XJ, Xiang WS (2014) Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora. Antonie Van Leeuwenhoek 105:307–315PubMedCrossRefGoogle Scholar
  8. Gordon RE, Barnett DA, Handerhan JE, Pang C (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63CrossRefGoogle Scholar
  9. Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65:501–509CrossRefGoogle Scholar
  10. Huss VAR, Festl H, Schleifer KH (1983) Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192PubMedCrossRefGoogle Scholar
  11. Jones KL (1949) Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145PubMedCentralPubMedGoogle Scholar
  12. Kelly KL (1964) Inter-society color council-national bureau of standard color-name charts 204 illustrated with centroid colors. US Government Printing Office, WashingtonGoogle Scholar
  13. Kim SB, Brown R, Oldfield C, Gilbert SC, Iliarionov S, Goodfellow M (2000) Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 50:2031–2036PubMedCrossRefGoogle Scholar
  14. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120PubMedCrossRefGoogle Scholar
  15. Lechevalier MP, Lechevalier HA (1980) The chemotaxonomy of actinomycetes. In: Dietz A, Thayer DW (eds) Actinomycete taxonomy special publication, vol 6. Society of Industrial Microbiology, Arlington, pp 227–291Google Scholar
  16. Lee YK, Kim HW, Liu CL, Lee HK (2003) A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52:245–250PubMedCrossRefGoogle Scholar
  17. Loqman S, Barka EA, Clément C, Ouhdouch Y (2009) Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. World J Microbiol Biotechnol 25:81–91CrossRefGoogle Scholar
  18. Mandel M, Marmur J (1968) Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206Google Scholar
  19. McKerrow J, Vagg S, McKinney T, Seviour EM, Maszenan AM, Brooks P, Seviour RJ (2000) A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 30:178–182PubMedCrossRefGoogle Scholar
  20. Mingma R, Duangmal K, Trakulnaleamsai S, Thamchaipenet A, Matsumoto A, Takahashi Y (2014) Sphaerisporangium rufum sp. nov., an endophytic actinomycete from roots of Oryza sativa L. Int J Syst Evol Microbiol 64:1077–1082PubMedCrossRefGoogle Scholar
  21. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233CrossRefGoogle Scholar
  22. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  23. Sagare AP, Lee YL, Lin TC, Chen CC, Tsay HS (2000) Cytokinin-induced somatic embryogenesis and plant regeneration in Corydalis yanhusuo (Fumariaceae)-a medicinal plant. Plant Sci 160:139–147PubMedCrossRefGoogle Scholar
  24. Saitou N, Ne M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  25. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340CrossRefGoogle Scholar
  26. Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 607–654Google Scholar
  27. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRefGoogle Scholar
  28. Uchida K, Kudo T, Suzuki K, Nakase T (1999) A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56PubMedCrossRefGoogle Scholar
  29. Wang XJ, Zhao JW, Liu CX, Wang JD, Shen Y, Jia FY, Wang L, Zhang J, Yu C, Xiang WS (2013) Nonomuraea solani sp. nov., a novel actinomycete isolated from eggplant root (Solanum melongena L.). Int J Syst Evol Microbiol 63:2418–2423PubMedCentralPubMedCrossRefGoogle Scholar
  30. Wayne LG et al (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  31. Wu C, Lu X, Qin M, Wang Y, Ruan J (1989) Analysis of menaquinone compound in microbial cells by HPLC. Microbiology 16:176–178Google Scholar
  32. Xiang WS, Liu CX, Wang XJ, Du J, Xi LJ, Huang Y (2011) Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 61:1165–1169PubMedCrossRefGoogle Scholar
  33. Xing J, Liu CX, Zhang YJ, He HR, Zhou Y, Li L, Zhao JW, Liu SH, Wang XJ, Xiang WS (2015) Sphaerisporangium dianthi sp. nov., an endophytic actinomycete isolated from a root of Dianthus chinensis L. Antonie Van Leeuwenhoek 107:9–14PubMedCrossRefGoogle Scholar
  34. Yokota A, Tamura T, Hasegawa T, Huang LH (1993) Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 43:805–812CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Xin Wang
    • 1
  • Chongxi Liu
    • 1
  • Jiaxu Cheng
    • 2
  • Yuejing Zhang
    • 1
  • Zhaoxu Ma
    • 1
  • Lianjie Li
    • 1
  • Xiangjing Wang
    • 1
  • Wensheng Xiang
    • 1
    • 2
  1. 1.Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education CommitteeNortheast Agricultural UniversityHarbinPeople’s Republic of China
  2. 2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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