Antonie van Leeuwenhoek

, Volume 108, Issue 1, pp 127–132 | Cite as

Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm

Original Paper

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, designated UST20140214-015BT, was isolated from a marine flatworm (Polyclad). The bacterium was found to be Gram-negative, oxidase and catalase positive, long-rod shaped, and motile by means of several peritrichous or subpolar flagella. Strain UST20140214-015BT grew optimally at 28 °C, at pH 7.0 and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain UST20140214-015BT belongs to the genus Pseudovibrio, with highest sequence similarity to Pseudovibrio japonicus WSF2T (96.9 %), followed by P. ascidiaceicola F423T (96.7 %), P. denitrificans DN34T (96.6 %), P. axinellae Ad2T (96.3 %). All the other species shared <95.5 % sequence similarity. The principal fatty acids were identified as summed feature 8 (C18:1ω7c/ω6c, as defined by the MIDI system; 70.8 %), C16:0 (9.1 %), summed feature 3 (C16:1ω6c/C16:1ω7c; 5.7 %). The G+C content of the chromosomal DNA was determined to be 53.3 mol%. The combined genotypic and phenotypic data show that strain UST20140214-015BT represents a novel species within the genus Pseudovibrio, for which the name Pseudovibrio hongkongensis sp. nov. is proposed, with the type strain UST20140214-015BT (=KCTC 42383T = MCCC 1K00451T).

Keywords

Pseudovibrio hongkongensis sp. nov. Flatworm Taxonomy 

Abbreviations

MCCC

Marine Culture Collection of China

KCTC

Korean Collection for Type Cultures

Notes

Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (863 program, No. 2012AA092103) and Seed Funding from Scientific and Technical Innovation Council of Shenzhen Government (Grant No. 827000012).

Supplementary material

10482_2015_470_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)
10482_2015_470_MOESM2_ESM.docx (157 kb)
Supplementary material 2 (DOCX 157 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Ying Xu
    • 1
    • 2
  • Qi Li
    • 2
  • Renmao Tian
    • 2
  • Qiliang Lai
    • 3
  • Yu Zhang
    • 1
  1. 1.Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life ScienceShenzhen UniversityShenzhenChina
  2. 2.Division of Life ScienceThe Hong Kong University of Science and TechnologyKowloonChina
  3. 3.Key Laboratory of Marine Genetic ResourcesThe Third Institute of State Oceanic AdministrationXiamenChina

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