Antonie van Leeuwenhoek

, Volume 107, Issue 6, pp 1451–1473 | Cite as

New Ceratocystis species from Eucalyptus and Cunninghamia in South China

  • FeiFei Liu
  • Michael Mbenoun
  • Irene Barnes
  • Jolanda Roux
  • Michael J. Wingfield
  • GuoQing Li
  • JieQiong Li
  • ShuaiFei Chen
Original Paper

Abstract

During routine surveys for possible fungal pathogens in the rapidly expanding plantations of Eucalyptus and Cunninghamia lanceolata in China, numerous isolates of unknown species in the genus Ceratocystis (Microascales) were obtained from tree wounds. In this study we identified the Ceratocystis isolates from Eucalyptus and Cunninghamia in the GuangDong, GuangXi, FuJian and HaiNan Provinces of South China based on morphology and through comparisons of DNA sequence data for the ITS, partial β-tubulin and TEF-1α gene regions. Morphological and DNA sequence comparisons revealed two previously unknown species residing in the Indo-Pacific Clade. These are described here as Ceratocystis cercfabiensis sp. nov. and Ceratocystiscollisensis sp. nov. Isolates of Ceratocystis cercfabiensis showed intragenomic variation in their ITS sequences and four strains were selected for cloning of the ITS gene region. Twelve ITS haplotypes were obtained from 17 clones selected for sequencing, differing in up to seven base positions and representing two separate phylogenetic groups. This is the first evidence of multiple ITS types in isolates of Ceratocystis residing in the Indo-Pacific Clade. Caution should thus be exercised when using the ITS gene region as a barcoding marker for Ceratocystis species in this clade. This study also represents the first record of a species of Ceratocystis from Cunninghamia.

Keywords

Ceratocystidaceae Fungal barcoding genes Multiple ITS types Plantation forestry 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.China Eucalypt Research Centre (CERC)Chinese Academy of Forestry (CAF)ZhanJiangChina
  3. 3.Department of Genetics, Forestry and Agricultural Research Institute (FABI)University of PretoriaPretoriaSouth Africa

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