Antonie van Leeuwenhoek

, Volume 107, Issue 6, pp 1437–1444 | Cite as

Lysobacter fragariae sp. nov. and Lysobacterrhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant

  • Hina Singh
  • Juan Du
  • Hien T. T. Ngo
  • KyungHwa Won
  • Jung-Eun Yang
  • Ki-Young Kim
  • Tae-Hoo Yi
Original Paper

Abstract

Two bacterial strains, designated THG-DN8.7T and THG-DN8.3T, were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7T had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407T (97.2 %), Lysobacter niabensis KACC 11587T (97.0 %) and Lysobacter oryzae KCTC 22249T (96.9 %), while strain THG-DN8.3T had closely similarity with L. niabensis KACC 11587T (98.1 %), L. oryzae KCTC 22249T (97.1 %) and L. yangpyeongensis KACC 11407T (96.1 %). DNA–DNA relatedness values between strains THG-DN8.7T and THG-DN8.3T and their closest phylogenetically neighbours were below 30.0 %, which indicates that strains THG-DN8.7T and THG-DN8.3T represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C15:0, iso-C16:0 and iso-C17:1ω9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7T and THG-DN8.3T were determined to be 66.9 and 67.8 mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7T and THG-DN8.3T to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7T (=KCTC 42236T = JCM 30322T) and THG-DN8.3T (=KCTC 42237T = JCM 30321T), respectively.

Keywords

Lysobacter fragariae Lysobacter rhizosphaerae Gram-negative 16S rRNA Ubiquinone-8 

Notes

Acknowledgments

This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).

Supplementary material

10482_2015_439_MOESM1_ESM.pdf (20 kb)
Supplementary material 1 (PDF 20 kb)
10482_2015_439_MOESM2_ESM.pdf (102 kb)
Supplementary material 2 (PDF 102 kb)
10482_2015_439_MOESM3_ESM.pdf (151 kb)
Supplementary material 3 (PDF 151 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Hina Singh
    • 1
  • Juan Du
    • 1
  • Hien T. T. Ngo
    • 1
  • KyungHwa Won
    • 1
  • Jung-Eun Yang
    • 1
  • Ki-Young Kim
    • 2
  • Tae-Hoo Yi
    • 1
  1. 1.Department of Oriental Medicine Biotechnology, College of Life ScienceKyung Hee UniversityYongin-siRepublic of Korea
  2. 2.Department of Genetic Engineering, College of Life ScienceKyung Hee UniversityYongin-siRepublic of Korea

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