Antonie van Leeuwenhoek

, Volume 107, Issue 6, pp 1411–1419 | Cite as

Hymenobacter humi sp. nov., a bacterium isolated from soil

  • Sathiyaraj Srinivasan
  • Eun Sun Joo
  • Jae-Jin Lee
  • Myung Kyum Kim
Original Paper

Abstract

A red-pink coloured, Gram-negative, rod-shaped bacterium designated as strain DG31AT was isolated from soil collected in Seoul, South Korea. The isolate was found to grow optimally at 25 °C on R2A agar. The highest degrees of 16S rRNA gene sequence similarities of the strain were found with Hymenobacter arizonensis JCM 13504T (98.0 %), Hymenobacter glaciei VUG-A130T (96.1 %), Hymenobacter soli PB17T (95.2 %), Hymenobacter antarcticus VUG-A42aaT (94.7 %) and Hymenobacterchitinivorans Txc1T (92.8 %). The DNA G+C content of the novel strain, DG31AT, was determined to be 60.8 mol%. Chemotaxonomic data revealed that the major fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c; 26.7 %), C16:1ω5c (18.9 %) and anteiso-C15:0 (12.9 %); the major polar lipid was identified as phosphatidylethanolamine; the polyamine pattern was found to contain sym-homospermidine; and the major quinone was identified as MK-7. The DNA–DNA relatedness of strain DG31AT with respect to H.arizonensis JCM 13504T was 19.5 ± 2.9 % (reciprocal, 19.3 ± 0.6 %). Based on these data, strain DG31AT should be classified within the genus Hymenobacter as a novel species for which the name Hymenobacter humi sp. nov. is proposed, with the type strain DG31AT (=KCTC 32523T = JCM 19635T).

Keywords

Cytophagaceae Hymenobacter Taxonomy 

Notes

Acknowledgments

We thank Dr. Aidan C. Parte for checking the etymology of the species name. This work was supported by a special research grant from Seoul Women’s University (2015).

Supplementary material

10482_2015_436_MOESM1_ESM.docx (236 kb)
Supplementary material 1 (DOCX 236 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Sathiyaraj Srinivasan
    • 1
  • Eun Sun Joo
    • 1
  • Jae-Jin Lee
    • 1
  • Myung Kyum Kim
    • 1
  1. 1.Department of Bio & Environmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulKorea

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