Streptomyces gilvigriseus sp. nov., a novel actinobacterium isolated from mangrove forest soil
- 476 Downloads
A novel Streptomyces, strain MUSC 26T, was isolated from mangrove soil at Tanjung Lumpur, Malaysia. The bacterium was observed to be Gram-positive and to form grayish yellow aerial and substrate mycelium on ISP 7 agar. A polyphasic approach was used to study the taxonomy of strain MUSC 26T, which shows a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. The cell wall peptidoglycan was determined to contain ll-diaminopimelic acid. The predominant menaquinones were identified as MK-9 (H8) and MK-9(H6). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylmethylethanolamine and hydroxyphosphatidylmethylethanolamine. The predominant cellular fatty acids (>10.0 %) were identified as anteiso-C15:0 (31.4 %), iso-C16:0 (16.3 %), iso-C15:0 (13.9 %) and anteiso-C17:0 (12.6 %). The cell wall sugars were found to be galactose, glucose, mannose, ribose and rhamnose. These results suggest that MUSC 26T should be placed within the genus Streptomyces. Phylogenetic analysis indicated that closely related strains include Streptomyces qinglanensis 172205T (96.5 % sequence similarity), S. sodiiphilus YIM 80305T (96.5 %) and S. rimosus subsp. rimosus ATCC 10970T (96.4 %). DNA–DNA relatedness values between MUSC 26T and closely related type strains ranged from 17.0 ± 2.2 to 33.2 ± 5.3 %. Comparison of BOX-PCR fingerprints indicated MUSC 26T presents a unique DNA profile. The DNA G+C content was determined to be 74.6 mol%. Based on this polyphasic study of MUSC 26T, it is concluded that this strain represents a novel species, for which the name Streptomyces gilvigriseus sp. nov. is proposed. The type strain is MUSC 26T (=DSMZ 42173T = MCCC 1K00504T).
KeywordsStreptomyces gilvigriseus sp. nov Actinobacteria Mangrove forest
This work was supported by a University of Malaya for High Impact Research Grant (UM-MOHE HIR Nature Microbiome Grant No. H-50001-A000027) awarded to K.-G. C. and an External Industry Grant from Biotek Abadi Sdn Bhd (vote nos. GBA-808138 & GBA-808813) awarded to L.-H. L. The authors are thankful to Professor Bernhard Schink for the support in the Latin etymology of the new species name.
- Atlas RM, Parks LC (eds) (1993) Handbook of microbiological media. CRC Press, Boca RatonGoogle Scholar
- Kates M (1986) Techniques of lipidology, 2nd edn. Elsevier, AmsterdamGoogle Scholar
- Kelly KL (1964) Inter-Society Color Council-National Bureau of standards color name charts illustrated with centroid colors. US Government Printing Office, Washington, DCGoogle Scholar
- Lee L-H, Zainal N, Azman A-S, Eng S-K, Goh B-H, Yin W-F, Ab Mutalib N-S, Chan K-G (2014c) Diversity and antimicrobial activities of actinobacteria isolated from tropical mangrove sediments in Malaysia. Sci World J 2014:14Google Scholar
- MacFaddin JF (2000) Biochemical tests for identification of medical bacteria, 3rd edn. Lippincott, Williams and Wilkins, BaltimoreGoogle Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. MIDI Inc, NewarkGoogle Scholar
- Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology. Williams & Wilkins, Baltimore, pp 2452–2492Google Scholar
- Yan XC (1992) Classification and identification of actinomycetes. Academic Press, BeijingGoogle Scholar