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Antonie van Leeuwenhoek

, Volume 107, Issue 6, pp 1369–1378 | Cite as

Streptomyces gilvigriseus sp. nov., a novel actinobacterium isolated from mangrove forest soil

  • Hooi-Leng Ser
  • Nurullhudda Zainal
  • Uma Devi Palanisamy
  • Bey-Hing Goh
  • Wai-Fong Yin
  • Kok-Gan Chan
  • Learn-Han LeeEmail author
Original Paper

Abstract

A novel Streptomyces, strain MUSC 26T, was isolated from mangrove soil at Tanjung Lumpur, Malaysia. The bacterium was observed to be Gram-positive and to form grayish yellow aerial and substrate mycelium on ISP 7 agar. A polyphasic approach was used to study the taxonomy of strain MUSC 26T, which shows a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. The cell wall peptidoglycan was determined to contain ll-diaminopimelic acid. The predominant menaquinones were identified as MK-9 (H8) and MK-9(H6). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylmethylethanolamine and hydroxyphosphatidylmethylethanolamine. The predominant cellular fatty acids (>10.0 %) were identified as anteiso-C15:0 (31.4 %), iso-C16:0 (16.3 %), iso-C15:0 (13.9 %) and anteiso-C17:0 (12.6 %). The cell wall sugars were found to be galactose, glucose, mannose, ribose and rhamnose. These results suggest that MUSC 26T should be placed within the genus Streptomyces. Phylogenetic analysis indicated that closely related strains include Streptomyces qinglanensis 172205T (96.5 % sequence similarity), S. sodiiphilus YIM 80305T (96.5 %) and S. rimosus subsp. rimosus ATCC 10970T (96.4 %). DNA–DNA relatedness values between MUSC 26T and closely related type strains ranged from 17.0 ± 2.2 to 33.2 ± 5.3 %. Comparison of BOX-PCR fingerprints indicated MUSC 26T presents a unique DNA profile. The DNA G+C content was determined to be 74.6 mol%. Based on this polyphasic study of MUSC 26T, it is concluded that this strain represents a novel species, for which the name Streptomyces gilvigriseus sp. nov. is proposed. The type strain is MUSC 26T (=DSMZ 42173T = MCCC 1K00504T).

Keywords

Streptomyces gilvigriseus sp. nov Actinobacteria Mangrove forest 

Notes

Acknowledgments

This work was supported by a University of Malaya for High Impact Research Grant (UM-MOHE HIR Nature Microbiome Grant No. H-50001-A000027) awarded to K.-G. C. and an External Industry Grant from Biotek Abadi Sdn Bhd (vote nos. GBA-808138 & GBA-808813) awarded to L.-H. L. The authors are thankful to Professor Bernhard Schink for the support in the Latin etymology of the new species name.

Supplementary material

10482_2015_431_MOESM1_ESM.doc (4.5 mb)
Supplementary material 1 (DOC 4588 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Hooi-Leng Ser
    • 1
  • Nurullhudda Zainal
    • 1
    • 2
  • Uma Devi Palanisamy
    • 1
  • Bey-Hing Goh
    • 1
  • Wai-Fong Yin
    • 2
  • Kok-Gan Chan
    • 2
  • Learn-Han Lee
    • 1
    Email author
  1. 1.Jeffrey Cheah School of Medicine and Health SciencesMonash University MalaysiaBandar SunwayMalaysia
  2. 2.Division of Genetics and Molecular Biology, Faculty of Science, Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia

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